ArticleThe Journal of allergy and clinical immunology2014
Influence of seasonal exposure to grass pollen on local and peripheral blood IgE repertoires in patients with allergic rhinitis.
Article in The Journal of allergy and clinical immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Genetic background shapes the naïve IgM B cell receptor repertoire in mice.Frontiers in immunology · 2026Article
- Skin Staphylococcus aureus detection and relationship to atopic dermatitis outcomes using culture and metagenomic sequencing.Scientific reports · 2025Article
- Multi-omics in Allergic Rhinitis: Mechanism Dissection and Precision Medicine.Clinical reviews in allergy & immunology · 2025Review
- Clonal evolution and stereotyped sequences of human IgE lineages in aeroallergen-specific immunotherapy.The Journal of allergy and clinical immunology · 2023Article
- ViCloD, an interactive web tool for visualizing B cell repertoires and analyzing intraclonal diversities: application to human B-cell tumors.NAR genomics and bioinformatics · 2023Article
- B Lineage Cells and IgE in Allergic Rhinitis and CRSwNP and the Role of Omalizumab Treatment.American journal of rhinology & allergy · 2023Review
- TLR Signaling Pathway Gene Polymorphisms, Gene-Gene and Gene-Environment Interactions in Allergic Rhinitis.Journal of inflammation research · 2022Article
- Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2021Article
- Short- and Long-Lived Autoantibody-Secreting Cells in Autoimmune Neurological Disorders.Frontiers in immunology · 2021Review
- B Cell Mobilization, Dissemination, Fine Tuning of Local Antigen Specificity and Isotype Selection in Asthma.Frontiers in immunology · 2021Article
- Local Respiratory Allergy: From Rhinitis Phenotype to Disease Spectrum.Frontiers in immunology · 2021Review
- Allergen Immunotherapy for Local Respiratory Allergy.Current allergy and asthma reports · 2020Review
- Origins and clonal convergence of gastrointestinal IgEScience immunology · 2020Article
- Mosaic deletion patterns of the human antibody heavy chain gene locus shown by Bayesian haplotyping.Nature communications · 2019Article
- IgE Test in Secretions of Patients with Respiratory Allergy.Current allergy and asthma reports · 2018Review
- IgE repertoire and immunological memory: compartmental regulation and antibody function.International immunology · 2018Review
- Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells.Frontiers in immunology · 2018Article
- Local Clonal Diversification and Dissemination of B Lymphocytes in the Human Bronchial Mucosa.Frontiers in immunology · 2018Article
- Antibodies and superantibodies in patients with chronic rhinosinusitis with nasal polyps.The Journal of allergy and clinical immunology · 2017Article
- Hierarchical Clustering Can Identify B Cell Clones with High Confidence in Ig Repertoire Sequencing Data.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundPrevious studies of immunoglobulin gene sequences in patients with allergic diseases using low-throughput Sanger sequencing have limited the analytic depth for characterization of IgE repertoires.
objectivesWe used a high-throughput, next-generation sequencing approach to characterize immunoglobulin heavy-chain gene (IGH) repertoires in patients with seasonal allergic rhinitis (AR) with the aim of better understanding the underlying disease mechanisms.
methodsIGH sequences in matched peripheral blood and nasal biopsy specimens from nonallergic healthy control subjects (n = 3) and patients with grass pollen-related AR taken in season (n = 3) or out of season (n = 4) were amplified and pyrosequenced on the 454 GS FLX+ System.
resultsA total of 97,610 IGH (including 8,135 IgE) sequences were analyzed. Use of immunoglobulin heavy-chain variable region gene families 1 (IGHV1) and 5 (IGHV5) was higher in IgE clonotypic repertoires compared with other antibody classes independent of atopic status. IgE repertoires measured inside the grass pollen season were more diverse and more mutated (particularly in the biopsy specimens) and had more evidence of antigen-driven selection compared with those taken outside of the pollen season or from healthy control subjects. Clonal relatedness was observed for IgE between the blood and nasal biopsy specimens. Furthermore in patients with AR, but not healthy control subjects, we found clonal relatedness between IgE and IgG classes.
conclusionThis is the first report that exploits next-generation sequencing to determine local and peripheral blood IGH repertoires in patients with respiratory allergic disease. We demonstrate that natural pollen exposure was associated with changes in IgE repertoires that were suggestive of ongoing germinal center reactions. Furthermore, these changes were more often apparent in nasal biopsy specimens compared with peripheral blood and in patients with AR compared with healthy control subjects.
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