ArticlePloS one2014
Assessing reference genes for accurate transcript normalization using quantitative real-time PCR in pearl millet [Pennisetum glaucum (L.) R. Br].
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 50 citations in OpenAlex.
- Identification and Validation of Reference Genes for Reliable RT-qPCR Normalization inPlants (Basel, Switzerland) · 2026Article
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- Comprehensive analysis of housekeeping genes, tissue-specific genes, and dynamic regulation across developmental stages in pearl millet.BMC genomics · 2024Article
- Chromosome-scale pearl millet genomes reveal CLAMT1b as key determinant of strigolactone pattern and Striga susceptibility.Nature communications · 2024Article
- Transcriptomic analysis of methyl jasmonate treatment reveals gene networks involved in drought tolerance in pearl millet.Scientific reports · 2022Article
- A Selection of Reliable Reference Genes for Gene Expression Analysis in the Female and Male Flowers ofPlants (Basel, Switzerland) · 2022Article
- Evaluation of reference genes and characterization of the MYBs in xylem radial change of Chinese fir stem.Scientific reports · 2022Article
- Imaging Salt Uptake Dynamics in Plants Using PET.Scientific reports · 2019Article
- Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae).Scientific reports · 2019Article
- Gene Expression Maps in Plants: Current State and Prospects.Plants (Basel, Switzerland) · 2019Review
- Identification of reliable reference genes for qRT-PCR in the ephemeral plant Arabidopsis pumila based on full-length transcriptome data.Scientific reports · 2019Article
- Identification of suitable internal control genes for transcriptional studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Antibiotic-induced acceleration of type 1 diabetes alters maturation of innate intestinal immunity.eLife · 2018Article
- Expression pattern of salt tolerance-related genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Reference gene identification for reliable normalisation of quantitative RT-PCR data inPlant methods · 2018Article
- Article
- Reference Gene Selection for qRT-PCR Normalization Analysis in kenaf (Frontiers in plant science · 2017Article
- Article
- Reference genes for accurate gene expression analyses across different tissues, developmental stages and genotypes in rice for drought tolerance.Rice (New York, N.Y.) · 2016Article
- Selection of suitable reference genes for assessing gene expression in pearl millet under different abiotic stresses and their combinations.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pearl millet [Pennisetum glaucum (L.) R.Br.], a close relative of Panicoideae food crops and bioenergy grasses, offers an ideal system to perform functional genomics studies related to C4 photosynthesis and abiotic stress tolerance. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) provides a sensitive platform to conduct such gene expression analyses. However, the lack of suitable internal control reference genes for accurate transcript normalization during qRT-PCR analysis in pearl millet is the major limitation. Here, we conducted a comprehensive assessment of 18 reference genes on 234 samples which included an array of different developmental tissues, hormone treatments and abiotic stress conditions from three genotypes to determine appropriate reference genes for accurate normalization of qRT-PCR data. Analyses of Ct values using Stability Index, BestKeeper, ΔCt, Normfinder, geNorm and RefFinder programs ranked PP2A, TIP41, UBC2, UBQ5 and ACT as the most reliable reference genes for accurate transcript normalization under different experimental conditions. Furthermore, we validated the specificity of these genes for precise quantification of relative gene expression and provided evidence that a combination of the best reference genes are required to obtain optimal expression patterns for both endogeneous genes as well as transgenes in pearl millet.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.