ArticlePloS one2014
Epithelial sheet folding induces lumen formation by Madin-Darby canine kidney cells in a collagen gel.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- E-cadherin mediates apical membrane initiation site localisation during de novo polarisation of epithelial cavities.The EMBO journal · 2022Article
- Improvement of the cell viability of hepatocytes cultured in three-dimensional collagen gels using pump-free perfusion driven by water level difference.Scientific reports · 2022Article
- Dominant geometrical factors of collective cell migration in flexible 3D gelatin tube structures.Biophysical reports · 2022Article
- An overview of bio-actuation in collagen hydrogels: a mechanobiological phenomenon.Biophysical reviews · 2021Review
- Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization.Cancer science · 2018Article
- Emergent patterns of collective cell migration under tubular confinement.Nature communications · 2017Article
- Conformational plasticity of JRAB/MICAL-L2 provides "law and order" in collective cell migration.Molecular biology of the cell · 2016Article
- Three-dimensional morphogenesis of MDCK cells induced by cellular contractile forces on a viscous substrate.Scientific reports · 2015Article
- Activating transcription factor 5 enhances radioresistance and malignancy in cancer cells.Oncotarget · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-β1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-β1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement.
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