Evidence map›Paper›PMID 25170296›Full record

ArticleInternational journal of medical sciences2014

In vitro protease inhibition and cytotoxicity of Aspergillus fumigatus biomolecules secreted under long-term aerated conditions.

Valentina S Arsic Arsenijevic, Marina G Pekmezovic, Katarina M Rajkovic, Berislav P Vekic, Aleksandra M Barac, Suzana Tasic-Otasevic, Ljubica Dj Petkovic

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of medical sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 3 countries.

Valentina S Arsic Arsenijevic1. National Reference Medical Mycology Laboratory, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, dr Subotića 1, 11 000 Belgrade, Serbia.
Marina G Pekmezovic1. National Reference Medical Mycology Laboratory, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, dr Subotića 1, 11 000 Belgrade, Serbia.
Katarina M Rajkovic2. High Chemical and Technological School for Professional Studies, Kosančićeva 36, 37 000 Kruševac, Serbia.
Berislav P Vekic3. Clinical Centre "Dr Dragiša Mišović" Department of Surgery, Milana Tepića 1, 11 000 Belgrade, Serbia; ; 4. University of Alfa, Palmira Toljatija 3, 11 000 Belgrade, Serbia.
Aleksandra M Barac1. National Reference Medical Mycology Laboratory, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, dr Subotića 1, 11 000 Belgrade, Serbia.
Suzana Tasic-Otasevic5. Institute of Microbiology and Immunology, Faculty of Medicine, University of Nis, Bulevar Dr Zorana Djindjica 81, 18000 Nis, Serbia.
Ljubica Dj Petkovic6. Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia ; 7. Gynecology Department Grajska ulica 24, 9000 Murska Sobota, Slovenia.
University of Belgrade · RSTechnical University of Košice · SKUniversity Hospital Center Dr Dragiša Mišović · RSUniversity of Ljubljana · SIUniversity of Nis · RS

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The fatality rate of invasive aspergillosis (IA) is still very high, especially in prolonged and untreated pulmonary cases. Aspergillus fumigatus is the main causative agent of IA and investigation of its metabolites could provide valuable insight into virulence factor(s) associated with this organism. We evaluated the A. fumigatus culture filtrate (CF) products generated during short- and long-term aerated and non-aerated conditions and tested for (i) inhibition of cysteine or serine proteases and (ii) cytotoxicity. In addition, the mathematical model was determined using response surface methodology (RSM) to estimate the influence of different fermentation conditions on A. fumigatus CF characteristics, predict enzyme inhibition and make possible correlations with in vivo conditions. Biosynthesis of A. fumigatus low molecular weight proteinaceous products (from 6.4 to 15.4 kDa) was observed after 6 days of growth under aerated and alkaline conditions. Also, only these CFs showed significant reduction in cell lines survival (Caco-2 and WISH 35.6% and 54.6%, respectively). Obtained results provide solid starting point for further studies that would include: (i) detailed chemical characterization of A. fumigatus CF, (ii) activity relationships and in vivo correlation with pathogenicity of prolonged pulmonary IA and (iii) possible use of biomolecules as diagnostic or therapeutic markers.

Indexed as

AerobiosisAspergillosisAspergillus fumigatusCaco-2 CellsCell Line, TumorCulture MediaHumansIn Vitro TechniquesModels, TheoreticalPeptide HydrolasesCulture MediaPeptide HydrolasesAspergillus fumigatusculture filtratecysteine protease inhibitioncytotoxicityoxygen

Identifiers

PMID25170296
PMCPMC4147639
OpenAlexW2145044393

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.