ArticleBMC complementary and alternative medicine2014
Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology.
Article in BMC complementary and alternative medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Optimization of Enzyme-Assisted Ultrasonic Extraction of a Quercetin-Containing Crude Extract fromFoods (Basel, Switzerland) · 2026Article
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- Total flavones fromAnnals of medicine · 2023Article
- Identification of microRNAs from Medicinal PlantPlants (Basel, Switzerland) · 2021Article
- Bamboo leaf flavone changed the community of cecum microbiota and improved the immune function in broilers.Scientific reports · 2020Article
- Extraction of Flavonoids From Natural Sources Using Modern Techniques.Frontiers in chemistry · 2020Review
- Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves.Evidence-based complementary and alternative medicine : eCAM · 2019Article
- Application of Ionic Liquid-Based Ultrasonic-Assisted Extraction of Flavonoids from Bamboo Leaves.Molecules (Basel, Switzerland) · 2018Article
- Quantitative Analysis of Phenolic Acids and Flavonoids inJournal of analytical methods in chemistry · 2018Article
- Flavonoid Extract from Propolis Inhibits Cardiac Fibrosis Triggered by Myocardial Infarction through Upregulation of SIRT1.Evidence-based complementary and alternative medicine : eCAM · 2018Article
- Optimization of microwave-assisted extraction of zerumbone fromChemistry Central journal · 2017Article
- Comparative study on conventional, ultrasonication and microwave assisted extraction of γ-oryzanol from rice bran.Journal of food science and technology · 2016Article
- Comparative Evaluation of Different Extraction Techniques and Solvents for the Assay of Phytochemicals and Antioxidant Activity of Hashemi Rice Bran.Molecules (Basel, Switzerland) · 2015Article
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4 authors.
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Abstract
backgroundExtraction prior to component analysis is the primary step in the recovery and isolation of bioactive phytochemicals from plant materials.
methodsResponse surface methodology was applied to optimize ultrasound-assisted extraction conditions followed by ultra high performance liquid chromatography (UHPLC) to achieve high catechin, myricetin, and quercetin contents, and high antioxidant and anticancer activities in the curry leaf extracts. The antioxidant and anticancer activities of the leaf extracts were determined by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, respectively. The central composite experimental design (3-level, 3-factorial) was employed to consider the effects of ultrasonic power (80-150 W), temperature (40-80°C), and methanol dilution (40-80%) on the properties of the curry leaf extracts.
resultsIt was found that ultrasonic power of 145.49 W at 55.9°C with 80% methanol was the most appropriate set of conditions for the extraction of catechin, myricetin, and quercetin from curry leaves with the consequent high antioxidant activity. Using the optimum extraction conditions, the extraction yields of catechin, myricetin, and quercetin were 0.482, 0.517, and 0.394 mg/g DW, respectively, and the antioxidant activity was enhanced to 83%. The optimized extract showed more distinct anticancer activity against HeLa cancer cells in a concentration of 67.2 μg/mL (P < 0.01) without toxicity to normal cells.
conclusionsThe results indicated that the pharmaceutical quality of curry leaves could be improved significantly by optimizing the extraction process using response surface methodology.
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