ArticleScientific reports2014
Quantification of surface tension and internal pressure generated by single mitotic cells.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed.
- Optimization of Atomic Force Microscopy Cantilever Specifications for Pressure Measurement in Biological Tissues.Annals of biomedical engineering · 2026Article
- Real-Time Monitoring of Mass and Mechanical Properties in Single Cells and Multicellular Spheroids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Simulation-Based Microfluidic Deformation Mapping for Region-Dependent Apparent Young's Modulus Estimation of Single Cells.Biosensors · 2026Article
- A geometric-surface PDE model for cell-nucleus translocation through confinement.PLoS computational biology · 2026Article
- Advancing mechanobiology from single molecules to complex cellular systems.Nature nanotechnology · 2026Review
- Cells Dynamically Adapt Their Nuclear Volumes and Proliferation Rates During Single to Multicellular Transitions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A surface morphology-based inference method for the cell wall elasticity profile in tip-growing cells.PLoS computational biology · 2026Article
- Stress-dependent growth in breast cancer arises from a mechano-osmotic coupling and cell-sizing checkpoint.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cell divisions both challenge and refine tissue boundaries in the Drosophila embryo.Development (Cambridge, England) · 2026Article
- Mapping single-cell rheology of ascidian embryos in the cleavage stages using AFM.Biophysical journal · 2025Article
- 3D multiscale shape analysis of nuclei and in vivo elastic stress sensors allows force inference.Biophysical journal · 2025Article
- Profiling the effect of low frequency mechanical vibration on the metabolic and oxidative stress responses of A431 carcinoma.FEBS open bio · 2025Article
- Delamination of epithelia induced by air-liquid interfaces.Molecular biology of the cell · 2025Article
- Atomic Force Microscopy Reveals Differences In Mechanical Properties Linked To Cortical Structure In Mouse And Human Oocytes.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- N-Cadherin based adhesion and Rac1 activity regulate tension polarization in the actin cortex.Scientific reports · 2025Article
- Intracellular pressure controls the propagation of tension in crumpled cell membranes.Nature communications · 2025Article
- The F-actin bundler SWAP-70 promotes tumor metastasis.Life science alliance · 2024Article
- Cell-intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Research progress on the regulatory role of cell membrane surface tension in cell behavior.Heliyon · 2024Review
- Quantitative comparison of cell-cell detachment force in different experimental setups.The European physical journal. E, Soft matter · 2024Article
40 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During mitosis, adherent cells round up, by increasing the tension of the contractile actomyosin cortex while increasing the internal hydrostatic pressure. In the simple scenario of a liquid cell interior, the surface tension is related to the local curvature and the hydrostatic pressure difference by Laplace's law. However, verification of this scenario for cells requires accurate measurements of cell shape. Here, we use wedged micro-cantilevers to uniaxially confine single cells and determine confinement forces while concurrently determining cell shape using confocal microscopy. We fit experimentally measured confined cell shapes to shapes obeying Laplace's law with uniform surface tension and find quantitative agreement. Geometrical parameters derived from fitting the cell shape, and the measured force were used to calculate hydrostatic pressure excess and surface tension of cells. We find that HeLa cells increase their internal hydrostatic pressure excess and surface tension from ≈ 40 Pa and 0.2 mNm(-1) during interphase to ≈ 400 Pa and 1.6 mNm(-1) during metaphase. The method introduced provides a means to determine internal pressure excess and surface tension of rounded cells accurately and with minimal cellular perturbation, and should be applicable to characterize the mechanical properties of various cellular systems.
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