ArticleAnnals of botany2014
Differential amplification of satellite PaB6 in chromosomally hypervariable Prospero autumnale complex (Hyacinthaceae).
Article in Annals of botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- From minor loci to major players? Satellite DNA diversification in Crepis sensu stricto.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Bread wheat satellitome: a complex scenario in a huge genome.Plant molecular biology · 2024Article
- Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes.The Plant journal : for cell and molecular biology · 2022Article
- The Chromosome Number and rDNA Loci Evolution inInternational journal of molecular sciences · 2022Article
- Repeat Dynamics across Timescales: A Perspective from Sibling Allotetraploid Marsh Orchids (Dactylorhiza majalis s.l.).Molecular biology and evolution · 2022Article
- Comparative repeatome analysis reveals new evidence on genome evolution in wild diploid Arachis (Fabaceae) species.Planta · 2022Article
- Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato.International journal of molecular sciences · 2022Article
- Morphological and Genome-Wide Evidence of Homoploid Hybridisation inPlants (Basel, Switzerland) · 2022Article
- Chromosomal view of Lippia alba, a tropical polyploid complex under genome stabilization process.Protoplasma · 2022Article
- Interstitial Telomeric-like Repeats (ITR) in Seed Plants as Assessed by Molecular Cytogenetic Techniques: A Review.Plants (Basel, Switzerland) · 2021Review
- Cytological Study ofPlants (Basel, Switzerland) · 2021Article
- Article
- The Evolution of Chromosome Numbers: Mechanistic Models and Experimental Approaches.Genome biology and evolution · 2021Article
- The Genomics of Plant Satellite DNA.Progress in molecular and subcellular biology · 2021Review
- Review
- Genome Size and Chromosome Number Evolution in KoreanPlants (Basel, Switzerland) · 2020Article
- Asymmetrical canina meiosis is accompanied by the expansion of a pericentromeric satellite in non-recombining univalent chromosomes in the genus Rosa.Annals of botany · 2020Article
- TheFrontiers in genetics · 2020Article
- Article
- Characterization of the satellitome in lower vascular plants: the case of the endangered fern Vandenboschia speciosa.Annals of botany · 2019Article
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7 authors.
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Abstract
BACKGROUND AND
aimsChromosomal evolution, including numerical and structural changes, is a major force in plant diversification and speciation. This study addresses genomic changes associated with the extensive chromosomal variation of the Mediterranean Prospero autumnale complex (Hyacinthaceae), which includes four diploid cytotypes each with a unique combination of chromosome number (x = 5, 6, 7), rDNA loci and genome size.
methodsA new satellite repeat PaB6 has previously been identified, and monomers were reconstructed from next-generation sequencing (NGS) data of P. autumnale cytotype B(6)B(6) (2n = 12). Monomers of all other Prospero cytotypes and species were sequenced to check for lineage-specific mutations. Copy number, restriction patterns and methylation levels of PaB6 were analysed using Southern blotting. PaB6 was localized on chromosomes using fluorescence in situ hybridization (FISH). KEY
resultsThe monomer of PaB6 is 249 bp long, contains several intact and truncated vertebrate-type telomeric repeats and is highly methylated. PaB6 is exceptional because of its high copy number and unprecedented variation among diploid cytotypes, ranging from 10(4) to 10(6) copies per 1C. PaB6 is always located in pericentromeric regions of several to all chromosomes. Additionally, two lineages of cytotype B(7)B(7) (x = 7), possessing either a single or duplicated 5S rDNA locus, differ in PaB6 copy number; the ancestral condition of a single locus is associated with higher PaB6 copy numbers.
conclusionsAlthough present in all Prospero species, PaB6 has undergone differential amplification only in chromosomally variable P. autumnale, particularly in cytotypes B(6)B(6) and B(5)B(5). These arose via independent chromosomal fusions from x = 7 to x = 6 and 5, respectively, accompanied by genome size increases. The copy numbers of satellite DNA PaB6 are among the highest in angiosperms, and changes of PaB6 are exceptionally dynamic in this group of closely related cytotypes of a single species. The evolution of the PaB6 copy numbers is discussed, and it is suggested that PaB6 represents a recent and highly dynamic system originating from a small pool of ancestral repeats.
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