ArticleInvestigative ophthalmology & visual science2014
Expression analysis of the transmembrane mucin MUC20 in human corneal and conjunctival epithelia.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Integrative Single-Cell Transcriptomic, Mendelian Randomization and In Silico Perturbation Analyses Prioritize MUC20 as a Candidate Gene Associated with Osteoporosis and Metabolic Dysfunction-Associated Steatotic Liver Disease in the Liver-Bone Axis.International journal of molecular sciences · 2026Article
- Evaluating Cytocompatibility of Corynebacterium glutamicum-poly Vinyl Alcohol Living Biomaterials for Ocular Use.Advanced healthcare materials · 2026Article
- The role of epithelial membrane-associated mucin 4 in ocular surface health and corneal wound healing.Frontiers in medicine · 2025Article
- PMToxicology and applied pharmacology · 2024Article
- Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model.Investigative ophthalmology & visual science · 2024Article
- Predicting Risks of Dry Eye Disease Development Using a Genome-Wide Polygenic Risk Score Model.Translational vision science & technology · 2024Article
- A framework for the simulation of individual glycan coordinates to analyze spatial relationships within the glycocalyx.Frontiers in cell and developmental biology · 2024Article
- The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.The ocular surface · 2023Article
- Single-cell RNA sequencing: Inhibited Notch2 signalling underlying the increased lens fibre cells differentiation in high myopia.Cell proliferation · 2023Article
- Comparison of mucin levels at the ocular surface of visual display terminal users with and without dry eye disease.BMC ophthalmology · 2023Article
- Diquafosol ophthalmic solution enhances mucin expression via ERK activation in human conjunctival epithelial cells with hyperosmotic stress.Molecular vision · 2022Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Membrane-associated mucins of the human ocular surface in health and disease.The ocular surface · 2021Review
- Identification of oral squamous cell carcinoma markers MUC2 and SPRR1B downstream of TANGO.Journal of cancer research and clinical oncology · 2021Article
- Optimization of an oral mucosaTissue barriers · 2020Article
- Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.Progress in retinal and eye research · 2020Review
- Selecting Appropriate Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Studies in Isolated and Cultured Ocular Surface Epithelia.Scientific reports · 2019Article
- Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.Carbohydrate research · 2018Article
- Glycosylation pathways at the ocular surface.Biochemical Society transactions · 2018Review
- TFOS DEWS II Tear Film Report.The ocular surface · 2017Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
purposeCell surface mucins are a group of highly O-glycosylated transmembrane glycoproteins responsible for the protection of epithelial cells on mucosal surfaces. The aim of this study was to investigate the localization and regulation of mucin 20 (MUC20) at the ocular surface.
methodsLocalization of MUC20 in human corneal and conjunctival epithelia was evaluated by immunofluorescence microscopy. Immortalized corneal (HCLE) and conjunctival (HCjE) cell lines were grown at different stages of differentiation and subjected to quantitative PCR and Western blot analyses. Cell surface proteins on apical cell membranes were biotinylated and isolated by neutravidin chromatography.
resultsThe MUC20 was detected throughout the entire human ocular surface epithelia, predominantly in cell membranes within intermediate cell layers. In conjunctiva, MUC20 also was observed in the cytoplasm of apical cells within the stratified squamous epithelium, but not in goblet cells. Quantitative PCR and immunoblotting demonstrated expression of MUC20 in HCLE and HCjE cells. Induction of differentiation with serum-containing medium resulted in upregulation of MUC20 mRNA and protein. Biotin labeling of the surface of stratified cultures revealed low levels of MUC20 protein on apical glycocalyces. Further, MUC20 was not detected in the cell culture media or in human tears, suggesting that the extracellular domain of MUC20 is not released from the ocular surface as described previously for other cell surface mucins.
conclusionsOur results indicate that MUC20 is a novel transmembrane mucin expressed by the human corneal and conjunctival epithelia, and suggest that differential expression of MUC20 during differentiation has a role in maintaining ocular surface homeostasis.
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