ArticleJournal of industrial microbiology & biotechnology2014
Expression of scorpion toxin LqhIT2 increases the virulence of Metarhizium acridum towards Locusta migratoria manilensis.
Article in Journal of industrial microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- MaAzaR Influences Virulence ofJournal of fungi (Basel, Switzerland) · 2024Article
- Expressing Parasitoid Venom Protein VRF1 in an EntomopathogenApplied and environmental microbiology · 2023Article
- Efficacy of genetically transformedSaudi journal of biological sciences · 2023Article
- Enhancing the Biocontrol Potential of the Entomopathogenic Fungus in Multiple Respects via the Overexpression of a Transcription Factor GeneJournal of fungi (Basel, Switzerland) · 2022Article
- Expression and purification of recombinant alpha-toxin AnCra1 from the scorpion Androctonus crassicauda and its functional characterization on mammalian sodium channels.Molecular biology reports · 2021Article
- High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris.Microbial cell factories · 2018Article
- Mode of Infection of Metarhizium spp. Fungus and Their Potential as Biological Control Agents.Journal of fungi (Basel, Switzerland) · 2017Review
- The unfulfilled promises of scorpion insectotoxins.The journal of venomous animals and toxins including tropical diseases · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LqhIT2 is an insect-specific neurotoxin from the venom of scorpion. In this study, the LqhIT2 gene was introduced into the entomopathogenic fungus, Metarhizium acridum. The virulence of the genetically modified strain MaLqhIT2 was then evaluated against locusts (Locusta migratoria manilensis). Compared with the wild-type strain, the median lethal cell density (LC50) for MaLqhIT2 was a 22.6-fold lower, and the median times to death (LT50) for MaLqhIT2 were reduced by 30.3 and 29.6 %, respectively, after topical inoculation and injection. MaLqhIT2 also grew significantly faster in the hemolymph than wild-type strain. There were no significant differences in germination, appressorium formation and sporulation in locust carcasses between the MaLqhIT2 and wild-type strain. These results indicate that LqhIT2 increased the virulence of M. acridum towards locusts by shortening the in vivo infection period, without affecting cuticle penetration or conidia formation in the carcasses. LqhIT2 thus shows considerable potential for increasing fungal virulence against locusts.
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