ArticleCell stress & chaperones2015
An artificial HSE promoter for efficient and selective detection of heat shock pathway activity.
Article in Cell stress & chaperones, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A Matter of Degrees: Latitudinal Variation in the Transcriptional Response to High and Low Temperatures in an Estuarine Cnidarian.bioRxiv : the preprint server for biology · 2026Article
- Article
- Conservation of mRNA operon formation in control of the heat shock response in mammalian cells.Science advances · 2025Article
- Quantitative Comparison of HSF1 Activators.Molecular biotechnology · 2022Article
- PARP1 Might Substitute HSF1 to Reactivate Latent HIV-1 by Binding to Heat Shock Element.Cells · 2022Article
- Article
- Caffeine and MDMA (Ecstasy) Exacerbate ER Stress Triggered by Hyperthermia.International journal of molecular sciences · 2022Article
- Genetically Encoded Protein Thermometer Enables Precise Electrothermal Control of Transgene Expression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq.Marine biotechnology (New York, N.Y.) · 2020Article
- Inhibitors of HSP90 in melanoma.Apoptosis : an international journal on programmed cell death · 2020Review
- Regulation Between HSF1 Isoforms and HSPs Contributes to the Variation in Thermal Tolerance Between Two Oyster Congeners.Frontiers in genetics · 2020Article
- Synthetic immunity by remote control.Theranostics · 2020Review
- Isolation and functional characterization of three abiotic stress-inducible (Apx, Dhn and Hsc70) promoters from pearl millet (Pennisetum glaucum L.).Molecular biology reports · 2019Article
- RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress.BMC genomics · 2019Article
- Targeted heat activation of HSP promoters in the skin of mammalian animals and humans.Cell stress & chaperones · 2018Article
- HSF1 mediated stress response of heavy metals.PloS one · 2018Article
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Authors and funding
5 authors.
Funding
Abstract
Detection of cellular stress is of major importance for the survival of cells. During evolution, a network of stress pathways developed, with the heat shock (HS) response playing a major role. The key transcription factor mediating HS signalling activity in mammalian cells is the HS factor HSF1. When activated it binds to the heat shock elements (HSE) in the promoters of target genes like heat shock protein (HSP) genes. They are induced by HSF1 but in addition they integrate multiple signals from different stress pathways. Here, we developed an artificial promoter consisting only of HSEs and therefore selectively reacting to HSF-mediated pathway activation. The promoter is highly inducible but has an extreme low basal level. Direct comparison with the HSPA1A promoter activity indicates that heat-dependent expression can be fully recapitulated by isolated HSEs in human cells. Using this sensitive reporter, we measured the HS response for different temperatures and exposure times. In particular, long heat induction times of 1 or 2 h were compared with short heat durations down to 1 min, conditions typical for burn injuries. We found similar responses to both long and short heat durations but at completely different temperatures. Exposure times of 2 h result in pathway activation at 41 to 44 °C, whereas heat pulses of 1 min lead to a maximum HS response between 47 and 50 °C. The results suggest that the HS response is initiated by a combination of temperature and exposure time but not by a certain threshold temperature.
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