ArticlePLoS neglected tropical diseases2014
Optimized multilocus sequence typing (MLST) scheme for Trypanosoma cruzi.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Deep Sequencing of mHVRs for Trypanosoma cruzi Genotyping.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Endemic potential of Chagas disease in the U.S. southwest: updated surveillance of infected Triatomines from the U.S.-Mexico border region.Epidemiology and infection · 2025Article
- Genetic diversity ofCurrent research in parasitology & vector-borne diseases · 2025Article
- New insights intoFrontiers in immunology · 2024Review
- Wide reference databases for typing Trypanosoma cruzi based on amplicon sequencing of the minicircle hypervariable region.PLoS neglected tropical diseases · 2023Article
- PhyloQuant approach provides insights into Trypanosoma cruzi evolution using a systems-wide mass spectrometry-based quantitative protein profile.Communications biology · 2021Article
- Guide RNA Repertoires in the Main Lineages ofFrontiers in cellular and infection microbiology · 2021Article
- Differential gene expression of virulence factors modulates infectivity of TcI Trypanosoma cruzi strains.Parasitology research · 2020Article
- Article
- Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I.PLoS neglected tropical diseases · 2020Article
- Chagas Disease in the United States: a Public Health Approach.Clinical microbiology reviews · 2019Review
- Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates ofPathogens (Basel, Switzerland) · 2019Article
- Elucidating diversity in the class composition of the minicircle hypervariable region of Trypanosoma cruzi: New perspectives on typing and kDNA inheritance.PLoS neglected tropical diseases · 2019Article
- Dissecting the phyloepidemiology of Trypanosoma cruzi I (TcI) in Brazil by the use of high resolution genetic markers.PLoS neglected tropical diseases · 2018Article
- Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids.Parasites & vectors · 2018Article
- Introgression of the Kinetoplast DNA: An Unusual Evolutionary Journey inCurrent genomics · 2018Review
- Cytochrome c oxidase subunit 1 gene as a DNA barcode for discriminating Trypanosoma cruzi DTUs and closely related species.Parasites & vectors · 2017Article
- Molecular Diversity of Trypanosoma cruzi Detected in the Vector Triatoma protracta from California, USA.PLoS neglected tropical diseases · 2016Article
- Between a bug and a hard place: Trypanosoma cruzi genetic diversity and the clinical outcomes of Chagas disease.Expert review of anti-infective therapy · 2015Review
- Evolution of Trypanosoma cruzi: clarifying hybridisations, mitochondrial introgressions and phylogenetic relationships between major lineages.Memorias do Instituto Oswaldo Cruz · 2015Review
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14 authors.
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Abstract
Trypanosoma cruzi, the aetiological agent of Chagas disease possess extensive genetic diversity. This has led to the development of a plethora of molecular typing methods for the identification of both the known major genetic lineages and for more fine scale characterization of different multilocus genotypes within these major lineages. Whole genome sequencing applied to large sample sizes is not currently viable and multilocus enzyme electrophoresis, the previous gold standard for T. cruzi typing, is laborious and time consuming. In the present work, we present an optimized Multilocus Sequence Typing (MLST) scheme, based on the combined analysis of two recently proposed MLST approaches. Here, thirteen concatenated gene fragments were applied to a panel of T. cruzi reference strains encompassing all known genetic lineages. Concatenation of 13 fragments allowed assignment of all strains to the predicted Discrete Typing Units (DTUs), or near-clades, with the exception of one strain that was an outlier for TcV, due to apparent loss of heterozygosity in one fragment. Monophyly for all DTUs, along with robust bootstrap support, was restored when this fragment was subsequently excluded from the analysis. All possible combinations of loci were assessed against predefined criteria with the objective of selecting the most appropriate combination of between two and twelve fragments, for an optimized MLST scheme. The optimum combination consisted of 7 loci and discriminated between all reference strains in the panel, with the majority supported by robust bootstrap values. Additionally, a reduced panel of just 4 gene fragments displayed high bootstrap values for DTU assignment and discriminated 21 out of 25 genotypes. We propose that the seven-fragment MLST scheme could be used as a gold standard for T. cruzi typing, against which other typing approaches, particularly single locus approaches or systematic PCR assays based on amplicon size, could be compared.
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