ArticleACS nano2014
Sensitive and selective plasmon ruler nanosensors for monitoring the apoptotic drug response in leukemia.
Article in ACS nano, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Novel Nanoplasmonic-Structure-Based Integrated Microfluidic Biosensors for Label-Free in Situ Immune Functional Analysis: A review of recent progress.IEEE nanotechnology magazine · 2020Article
- Plasmon Rulers as a Probe for Real-Time Microsecond Conformational Dynamics of Single Molecules.Nano letters · 2018Article
- Caspases and their substrates.Cell death and differentiation · 2017Review
- Collagen-Gold Nanoparticle Conjugates for Versatile Biosensing.Sensors (Basel, Switzerland) · 2017Article
- Monitoring transient nanoparticle interactions with liposome-confined plasmonic transducers.Microsystems & nanoengineering · 2017Article
- A Programmed Nanoparticle with Self-Adapting for Accurate Cancer Cell Eradication and Therapeutic Self-Reporting.Theranostics · 2017Article
- A dual-functional lanthanide nanoprobe for both living cell imaging and ICP-MS quantification of active protease.Chemical science · 2016Article
- Biofunctionalization of Selenium Nanoparticle with Dictyophora Indusiata Polysaccharide and Its Antiproliferative Activity through Death-Receptor and Mitochondria-Mediated Apoptotic Pathways.Scientific reports · 2015Article
- Article
- Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules.Nano letters · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Caspases are proteases involved in cell death, where caspase-3 is the chief executioner that produces an irreversible cutting event in downstream protein substrates and whose activity is desired in the management of cancer. To determine such activity in clinically relevant samples with high signal-to-noise, plasmon rulers are ideal because they are sensitively affected by their interparticle separation without ambiguity from photobleaching or blinking effects. A plasmon ruler is a noble metal nanoparticle pair, tethered in close proximity to one another via a biomolecule, that acts through dipole-dipole interactions and results in the light scattering to increase exponentially. In contrast, a sharp decrease in intensity is observed when the pair is confronted by a large interparticle distance. To align the mechanism of protease activity with building a sensor that can report a binary signal in the presence or absence of caspase-3, we present a caspase-3 selective plasmon ruler (C3SPR) composed of a pair of Zn0.4Fe2.6O4@SiO2@Au core-shell nanoparticles connected by a caspase-3 cleavage sequence. The dielectric core (Zn0.4Fe2.6O4@SiO2)-shell (Au) geometry provided a brighter scattering intensity versus solid Au nanoparticles, and the magnetic core additionally acted as a purification handle during the plasmon ruler assembly. By monitoring the decrease in light scattering intensity per plasmon ruler, we detected caspase-3 activity at single molecule resolution across a broad dynamic range. This was observed to be as low as 100 fM of recombinant material or 10 ng of total protein from cellular lysate. By thorough analyses of single molecule trajectories, we show caspase-3 activation in a drug-treated chronic myeloid leukemia (K562) cancer system as early as 4 and 8 h with greater sensitivity (2- and 4-fold, respectively) than conventional reagents. This study provides future implications for monitoring caspase-3 as a biomarker and efficacy of drugs.
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