ArticlePloS one2014
Overexpression of mitochondrial sirtuins alters glycolysis and mitochondrial function in HEK293 cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- BZD9L1 Differentially Regulates Sirtuins in Liver-Derived Cells by Inducing Reactive Oxygen Species.Biomedicines · 2023Article
- ITCH deficiency clinical phenotype expansion and mitochondrial dysfunction.Molecular genetics and metabolism reports · 2022Article
- Mitochondrial dysfunction associated with TANGO2 deficiency.Scientific reports · 2022Article
- Sirt1 and Sirt3 Activation Improved Cardiac Function of Diabetic Rats via Modulation of Mitochondrial Function.Antioxidants (Basel, Switzerland) · 2021Article
- Importance of Nutrient Availability and Metabolism for Skeletal Muscle Regeneration.Frontiers in physiology · 2021Review
- NADFrontiers in medicine · 2021Review
- The Role of Sirtuins in Kidney Diseases.International journal of molecular sciences · 2020Review
- Article
- Sirtuin 3 deficiency does not impede digit regeneration in mice.Scientific reports · 2019Article
- Reticular Dysgenesis and Mitochondriopathy Induced by Adenylate Kinase 2 Deficiency with Atypical Presentation.Scientific reports · 2019Article
- The fatty acid oxidation enzyme long-chain acyl-CoA dehydrogenase can be a source of mitochondrial hydrogen peroxide.Redox biology · 2019Article
- Von Hippel-Lindau Acts as a Metabolic Switch Controlling Nephron Progenitor Differentiation.Journal of the American Society of Nephrology : JASN · 2019Article
- Induction of Pyruvate Dehydrogenase Kinase 1 by Hypoxia Alters Cellular Metabolism and Inhibits Apoptosis in Endometriotic Stromal Cells.Reproductive sciences (Thousand Oaks, Calif.) · 2019Article
- Mitochondrial Function, Metabolic Regulation, and Human Disease Viewed through the Prism of Sirtuin 4 (SIRT4) Functions.Journal of proteome research · 2019Review
- Emerging role of SIRT3 in endothelial metabolism, angiogenesis, and cardiovascular disease.Journal of cellular physiology · 2019Review
- SIRT4 is essential for metabolic control and meiotic structure during mouse oocyte maturation.Aging cell · 2018Article
- Distinctive Roles of Sirtuins on Diabetes, Protective or Detrimental?Frontiers in endocrinology · 2018Review
- The Roles of Mitochondrial SIRT4 in Cellular Metabolism.Frontiers in endocrinology · 2018Review
- Mild metabolic perturbations alter succinylation of mitochondrial proteins.Journal of neuroscience research · 2017Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
SIRT3, SIRT4, and SIRT5 are mitochondrial deacylases that impact multiple facets of energy metabolism and mitochondrial function. SIRT3 activates several mitochondrial enzymes, SIRT4 represses its targets, and SIRT5 has been shown to both activate and repress mitochondrial enzymes. To gain insight into the relative effects of the mitochondrial sirtuins in governing mitochondrial energy metabolism, SIRT3, SIRT4, and SIRT5 overexpressing HEK293 cells were directly compared. When grown under standard cell culture conditions (25 mM glucose) all three sirtuins induced increases in mitochondrial respiration, glycolysis, and glucose oxidation, but with no change in growth rate or in steady-state ATP concentration. Increased proton leak, as evidenced by oxygen consumption in the presence of oligomycin, appeared to explain much of the increase in basal oxygen utilization. Growth in 5 mM glucose normalized the elevations in basal oxygen consumption, proton leak, and glycolysis in all sirtuin over-expressing cells. While the above effects were common to all three mitochondrial sirtuins, some differences between the SIRT3, SIRT4, and SIRT5 expressing cells were noted. Only SIRT3 overexpression affected fatty acid metabolism, and only SIRT4 overexpression altered superoxide levels and mitochondrial membrane potential. We conclude that all three mitochondrial sirtuins can promote increased mitochondrial respiration and cellular metabolism. SIRT3, SIRT4, and SIRT5 appear to respond to excess glucose by inducing a coordinated increase of glycolysis and respiration, with the excess energy dissipated via proton leak.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.