ArticleMolecular biology of the cell2014
Fractionation profiling: a fast and versatile approach for mapping vesicle proteomes and protein-protein interactions.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Clathrin-Mediated Endocytosis in Plants: Historical to Modern Advances.Traffic (Copenhagen, Denmark) · 2026Review
- Site-specific glycosylation of Sec24D and myoferlin recruit ERGIC to ER exit sites for collagen trafficking.Nature communications · 2026Article
- FGFR3 drives Aβ-induced tau uptake.Experimental & molecular medicine · 2024Article
- Cargo selective vesicle tethering: The structural basis for binding of specific cargo proteins by the Golgi tether component TBC1D23.Science advances · 2024Article
- Comparative Analysis of T-Cell Spatial Proteomics and the Influence of HIV Expression.Molecular & cellular proteomics : MCP · 2022Article
- PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1.Database : the journal of biological databases and curation · 2022Article
- Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.The EMBO journal · 2021Article
- Subcellular proteomics.Nature reviews. Methods primers · 2021Article
- Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers.Nature communications · 2020Article
- Organellar Maps Through Proteomic Profiling - A Conceptual Guide.Molecular & cellular proteomics : MCP · 2020Review
- Identification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles.Kidney360 · 2020Article
- Structure of TBC1D23 N-terminus reveals a novel role for rhodanese domain.PLoS biology · 2020Article
- Endosome-to-TGN Trafficking: Organelle-Vesicle and Organelle-Organelle Interactions.Frontiers in cell and developmental biology · 2020Review
- Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.Traffic (Copenhagen, Denmark) · 2019Article
- The expanding spectrum of neurological disorders of phosphoinositide metabolism.Disease models & mechanisms · 2019Review
- Spatial proteomics: a powerful discovery tool for cell biology.Nature reviews. Molecular cell biology · 2019Review
- AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A.Nature communications · 2018Article
- The WDR11 complex facilitates the tethering of AP-1-derived vesicles.Nature communications · 2018Article
- Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development.Scientific reports · 2018Article
- The two TRAPP complexes of metazoans have distinct roles and act on different Rab GTPases.The Journal of cell biology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
We developed "fractionation profiling," a method for rapid proteomic analysis of membrane vesicles and protein particles. The approach combines quantitative proteomics with subcellular fractionation to generate signature protein abundance distribution profiles. Functionally associated groups of proteins are revealed through cluster analysis. To validate the method, we first profiled >3500 proteins from HeLa cells and identified known clathrin-coated vesicle proteins with >90% accuracy. We then profiled >2400 proteins from Drosophila S2 cells, and we report the first comprehensive insect clathrin-coated vesicle proteome. Of importance, the cluster analysis extends to all profiled proteins and thus identifies a diverse range of known and novel cytosolic and membrane-associated protein complexes. We show that it also allows the detailed compositional characterization of complexes, including the delineation of subcomplexes and subunit stoichiometry. Our predictions are presented in an interactive database. Fractionation profiling is a universal method for defining the clathrin-coated vesicle proteome and may be adapted for the analysis of other types of vesicles and particles. In addition, it provides a versatile tool for the rapid generation of large-scale protein interaction maps.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.