ArticleThe Journal of biological chemistry2014
The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 55 citations in OpenAlex.
- Immune protection and persistence in Pseudomonas aeruginosa infections by pyruvate cross-feeding.Nature communications · 2026Article
- Decoding the spatiotemporal heterogeneity of bacterial virulence gene expression using single-cell approaches.Frontiers in microbiology · 2026Review
- Virulence Plasmid Modulates Glucose-Mediated Biofilm Regulation inLife (Basel, Switzerland) · 2025Article
- A master regulator of central carbon metabolism directly activates virulence gene expression in attaching and effacing pathogens.PLoS pathogens · 2024Article
- Enhanced Hemolytic Activity of MesophilicMicroorganisms · 2022Article
- The Biological Significance of Pyruvate Sensing and Uptake inMicroorganisms · 2022Article
- Article
- Insights into a Pyruvate Sensing and Uptake System inJournal of bacteriology · 2021Article
- Structure, Function and Regulation of a Second Pyruvate Kinase Isozyme inFrontiers in microbiology · 2021Article
- The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits ofFrontiers in microbiology · 2021Article
- Function and Regulation of the Pyruvate Transporter CstA inInternational journal of molecular sciences · 2020Article
- RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression.PLoS pathogens · 2020Article
- Evolutionary plasticity in the allosteric regulator-binding site of pyruvate kinase isoform PykA fromThe Journal of biological chemistry · 2019Article
- A bacterial secreted translocator hijacks riboregulators to control type III secretion in response to host cell contact.PLoS pathogens · 2019Article
- Article
- Metabolome and transcriptome-wide effects of the carbon storage regulator A in enteropathogenic Escherichia coli.Scientific reports · 2019Article
- The Manganese-Dependent Pyruvate Kinase PykM Is Required for Wild-Type Glucose Utilization by Brucella abortus 2308 and Its Virulence in C57BL/6 Mice.Journal of bacteriology · 2018Article
- Review
- BtsT, a Novel and Specific Pyruvate/HJournal of bacteriology · 2018Article
- Article
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Despite our increasing knowledge of the specific pathogenicity factors in bacteria, the contribution of metabolic processes to virulence is largely unknown. Here, we elucidate a tight connection between pathogenicity and core metabolism in the enteric pathogen Yersinia pseudotuberculosis by integrated transcriptome and [(13)C]fluxome analysis of the wild type and virulence-regulator mutants. During aerobic growth on glucose, Y. pseudotuberculosis reveals an unusual flux distribution with a high level of secreted pyruvate. The absence of the transcriptional and post-transcriptional regulators RovA, CsrA, and Crp strongly perturbs the fluxes of carbon core metabolism at the level of pyruvate metabolism and the tricarboxylic acid (TCA) cycle, and these perturbations are accompanied by transcriptional changes in the corresponding enzymes. Knock-outs of regulators of this metabolic branch point and of its central enzyme, pyruvate kinase (ΔpykF), result in mutants with significantly reduced virulence in an oral mouse infection model. In summary, our work identifies the pyruvate-TCA cycle node as a focal point for controlling the host colonization and virulence of Yersinia.
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