ArticleClinical oral investigations2015
Nitrogen-containing bisphosphonates modulate the antigenic profile and inhibit the maturation and biomineralization potential of osteoblast-like cells.
Article in Clinical oral investigations, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Dexamethasone Long-Term Controlled Release from Injectable Dual-Network Hydrogels with Porous Microspheres Immunomodulation Promotes Bone Regeneration.ACS applied materials & interfaces · 2024Article
- Biomimetic Collagen Membranes as Drug Carriers of Geranylgeraniol to Counteract the Effect of Zoledronate.Biomimetics (Basel, Switzerland) · 2023Article
- Bone Grafts in Dental Medicine: An Overview of Autografts, Allografts and Synthetic Materials.Materials (Basel, Switzerland) · 2023Review
- Nitrogen-Containing Bisphosphonates Downregulate Cathepsin K and Upregulate Annexin V in Osteoclasts CulturedInternational journal of dentistry · 2023Article
- Repercussions of Bisphenol A on the Physiology of Human Osteoblasts.International journal of molecular sciences · 2022Article
- Human adipose tissue-derived mesenchymal stromal cells and their phagocytic capacity.Journal of cellular and molecular medicine · 2022Article
- Zoledronic Acid Deteriorates Soft and Hard Tissue Healing of Murine Tooth Extraction Sockets in a Dose-Dependent Manner.Calcified tissue international · 2022Article
- Article
- Alendronate enhances osseointegration in a murine implant model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts.Polymers · 2021Article
- The Case of Medication-Related Osteonecrosis of the Jaw Addressed from a Pathogenic Point of View. Innovative Therapeutic Strategies: Focus on the Most Recent Discoveries on Oral Mesenchymal Stem Cell-Derived Exosomes.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Salivary Biomarkers and Their Application in the Diagnosis and Monitoring of the Most Common Oral Pathologies.International journal of molecular sciences · 2020Review
- Evaluation of bone turnover after bisphosphonate withdrawal and its influence on implant osseointegration: an in vivo study in rats.Clinical oral investigations · 2019Article
- Influence of pH on osteoclasts treated with zoledronate and alendronate.Clinical oral investigations · 2019Article
- Impact of bisphosphonates on the proliferation and gene expression of human fibroblasts.International journal of medical sciences · 2019Article
- Cytoprotective effect of flavonoid-induced autophagy on bisphosphonate mediated cell death in osteoblast.Journal of cellular biochemistry · 2018Article
- Bisphosphonate Modulation of the Gene Expression of Different Markers Involved in Osteoblast Physiology: Possible Implications in Bisphosphonate-Related Osteonecrosis of the Jaw.International journal of medical sciences · 2018Article
- Dose-Dependent Effects of Zoledronic Acid on Human Periodontal Ligament Stem Cells: AnCell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe aim was to evaluate the effect of three nitrogen-containing bisphosphonates at different concentrations on osteoblast growth, differentiation, and antigenic profile, using the MG-63 cell line as osteoblast model, in order to determine the role of osteoblasts in bisphosphonate-related osteonecrosis of the jaw (BRONJ). MATERIALS AND
methodsOsteoblasts were incubated in culture medium with 10(-5), 10(-7), or 10(-9) M of pamidronate, alendronate, or ibandronate. Proliferative capacity of the osteoblasts was determined by spectrophotometry (MTT) at 24 and 48 h of culture. Flow cytometry was used to study antigenic profile (CD54, CD80, CD86, HLA-DR) and phagocytic activity. Cell differentiation was evaluated at 7, 15, and 21 days by the study of nodule formation and alkaline phosphatase activity (ALP) at 24 h by spectrophotometric assay.
resultsPamidronate, alendronate, and ibandronate each exerted a significant stimulatory effect on MG63 proliferation that depended on the dose and treatment duration (p < 0.05). In general, a significantly decreased expression of CD54, CD80, and HLA-DR membrane antigens was observed after 24 h of treatment with each nitrogen-containing bisphosphonate (p < 0.05), but there was no significant difference in phagocytic activity versus controls. A decrease in ALP activity was observed after 24 h of treatment and a decrease in calcium deposition after 15 and 21 days (p < 0.05).
conclusionNitrogen-containing bisphosphonates can increase the proliferation of MG-63 osteoblast-like cells, modulate their expression of co-stimulatory molecules associated with immune function, and decrease their differentiation capacity, generally at low doses. CLINICAL RELEVANCE: These findings suggest that low doses of nitrogen-containing bisphosphonates exert their effect on osteoblasts by altering their physiology, which would explain the disruption of their repair capacity and may be directly related to the development of BRONJ.
Indexed as
Identifiers
25164156What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.