ArticleScientific reports2014
Stiff substrates enhance cultured neuronal network activity.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 85 citations in OpenAlex.
- In Vivo Direct Reprogramming: Current Progress and Future Prospects from Mechanisms to Therapeutic Application.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential.Advanced healthcare materials · 2026Review
- Neuromechanobiology: Bridging Mechanobiology and Neuroscience Through Evidence and Open Questions.Cells · 2026Review
- Environmental stiffness regulates neuronal maturation via Piezo1-mediated transthyretin activity.Nature communications · 2025Article
- Advances in large-scale electrophysiology with high-density microelectrode arrays.Lab on a chip · 2025Review
- Electrical and magnetic stimulation separately modulates the extent and direction of neurite outgrowth in an ionically conductive hydrogel.Journal of neural engineering · 2025Article
- A 3D Bioprinted Cortical Organoid Platform for Modeling Human Brain Development.Advanced healthcare materials · 2024Article
- Nanomechanical mapping of PLA hydroxyapatite composite scaffolds links surface homogeneity to stem cell differentiation.Scientific reports · 2024Article
- Mechanical stimulation and electrophysiological monitoring at subcellular resolution reveals differential mechanosensation of neurons within networks.Nature nanotechnology · 2024Article
- An optimized method of culturing neurons based on polyacrylamide gel.Biophysics reports · 2024Article
- In Vitro Pharmacological Modulation of PIEZO1 Channels in Frontal Cortex Neuronal Networks.Brain sciences · 2024Article
- HDAC6 inhibition regulates substrate stiffness-mediated inflammation signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2023Article
- Mechanical properties of the brain: Focus on the essential role of Piezo1-mediated mechanotransduction in the CNS.Brain and behavior · 2023Review
- Hippocampus of the APPFrontiers in aging neuroscience · 2023Article
- An overview of substrate stiffness guided cellular response and its applications in tissue regeneration.Bioactive materials · 2022Review
- Glutamate Receptors Mediate Changes to Dendritic Mitochondria in Neurons Grown on Stiff Substrates.Annals of biomedical engineering · 2022Article
- Substrate stiffness-dependent regulatory volume decrease and calcium signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2022Article
- Multiscale Mechanobiology in Brain Physiology and Diseases.Frontiers in cell and developmental biology · 2022Review
- Self-Assembling Polypeptide Hydrogels as a Platform to Recapitulate the Tumor Microenvironment.Cancers · 2021Article
- Understanding, engineering, and modulating the growth of neural networks: An interdisciplinary approach.Biophysics reviews · 2021Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanical property of extracellular matrix and cell-supporting substrates is known to modulate neuronal growth, differentiation, extension and branching. Here we show that substrate stiffness is an important microenvironmental cue, to which mouse hippocampal neurons respond and integrate into synapse formation and transmission in cultured neuronal network. Hippocampal neurons were cultured on polydimethylsiloxane substrates fabricated to have similar surface properties but a 10-fold difference in Young's modulus. Voltage-gated Ca(2+) channel currents determined by patch-clamp recording were greater in neurons on stiff substrates than on soft substrates. Ca(2+) oscillations in cultured neuronal network monitored using time-lapse single cell imaging increased in both amplitude and frequency among neurons on stiff substrates. Consistently, synaptic connectivity recorded by paired recording was enhanced between neurons on stiff substrates. Furthermore, spontaneous excitatory postsynaptic activity became greater and more frequent in neurons on stiff substrates. Evoked excitatory transmitter release and excitatory postsynaptic currents also were heightened at synapses between neurons on stiff substrates. Taken together, our results provide compelling evidence to show that substrate stiffness is an important biophysical factor modulating synapse connectivity and transmission in cultured hippocampal neuronal network. Such information is useful in designing instructive scaffolds or supporting substrates for neural tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.