ArticleAmerican journal of physiology. Cell physiology2014
Serum starvation regulates E-cadherin upregulation via activation of c-Src in non-small-cell lung cancer A549 cells.
Article in American journal of physiology. Cell physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Glycolytic suppression of cell adhesion promotes thyroid morphogenesis in response to nutrition.EMBO reports · 2026Article
- RPARP-AS1 Targets miR-10b-5p to Influence Prognosis in Non-Small Cell Lung Cancer and Inhibit Tumor Progression.Analytical cellular pathology (Amsterdam) · 2026Article
- Multi-omics reveals FAT10 as an immunometabolic survival factor rewiring global metabolism in cancer.Cell communication and signaling : CCS · 2025Article
- NDR2 regulates non-small cell lung cancer cell migration under starvation by supporting autophagosome biogenesis through LC3 and ATG9A regulation.Cell death discovery · 2025Article
- From Scarcity to Survival: Adaptive Strategies of A549 Lung Cancer Cells Under Serum Starvation.Thoracic research and practice · 2025Article
- Role of Lipopolysaccharides in the Inflammation and Pyroptosis of Alveolar Epithelial Cells in Acute Lung Injury and Acute Respiratory Distress Syndrome.Journal of inflammation research · 2024Review
- The Beneficial and Adverse Effects of Autophagic Response to Caloric Restriction and Fasting.Advances in nutrition (Bethesda, Md.) · 2023Review
- How Far Are We from Prescribing Fasting as Anticancer Medicine?International journal of molecular sciences · 2020Review
- Optimization of recombinant Zika virus NS1 protein secretion from HEK293 cells.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Serum-reduced media impacts on cell viability and protein expression in human lung epithelial cells.Journal of cellular physiology · 2019Article
- Clinical significance of epithelial-mesenchymal transition-related molecules in lung adenocarcinoma.Current oncology (Toronto, Ont.) · 2019Article
- Serum starvation induces cell death in NSCLC via miR-224.OncoTargets and therapy · 2019Article
- A novel mechanism of plasminogen activation in epithelial and mesenchymal cells.Scientific reports · 2018Article
- Regulation of transforming growth factor β-mediated epithelial-mesenchymal transition of lens epithelial cells by c-Src kinase under high glucose conditions.Experimental and therapeutic medicine · 2018Article
- The AFrontiers in pharmacology · 2018Article
- The deubiquitinating enzyme USP48 stabilizes TRAF2 and reduces E-cadherin-mediated adherens junctions.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Production of bFGF monoclonal antibody and its inhibition of metastasis in Lewis lung carcinoma.Molecular medicine reports · 2017Article
- A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry.Scientific reports · 2017Article
- Dietary restriction: could it be considered as speed bump on tumor progression road?Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Review
- Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway.International journal of molecular medicine · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
E-cadherin is essential for the integrity of adherens junctions between lung epithelial cells, and the loss of E-cadherin allows cell motility and is thought to promote lung cancer metastasis. While the downregulation of E-cadherin expression has been well characterized and is seen with transforming growth factor-β1 (TGF-β1) exposure, few studies have focused on E-cadherin upregulation. Here, we show that serum starvation causes increased E-cadherin expression via the activation of c-Src kinase in non-small-cell lung cancer A549 cells. Serum starvation increased E-cadherin protein levels in a time- and dose-dependent manner. E-cadherin mRNA transcripts were unchanged with starvation, while protein translation inhibition with cycloheximide attenuated E-cadherin protein induction by starvation, suggesting that E-cadherin is regulated at the translational level by serum starvation. c-Src is a nonreceptor tyrosine kinase known to regulate protein translation machinery; serum starvation caused early and sustained activation of c-Src in A549 cells followed by E-cadherin upregulation. Furthermore, overexpression of a dominant negative c-Src attenuated the induction of E-cadherin by serum deprivation. Finally, we observed that TGF-β1 treatment attenuated the serum activation of c-Src as well as E-cadherin expression when cells were deprived of serum. In conclusion, our data demonstrate that the c-Src kinase is activated by serum starvation to increase E-cadherin expression in A549 cells, and these phenomena are antagonized by TGF-β1. These novel observations implicate the c-Src kinase as an upstream inducer of E-cadherin protein translation with serum starvation and TGF-β1 diametrically regulating c-Src kinase activity and thus E-cadherin abundance in A549 cells.
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