ArticleMicrobial cell factories2014
Rational and combinatorial approaches to engineering styrene production by Saccharomyces cerevisiae.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 87 citations in OpenAlex.
- Microbial engineering for monocyclic aromatic compounds production.FEMS microbiology reviews · 2025Review
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- Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas.Microbial cell factories · 2024Article
- Styrene Production in Genetically EngineeredBiotech (Basel (Switzerland)) · 2024Article
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- Insights into the susceptibility of Pseudomonas putida to industrially relevant aromatic hydrocarbons that it can synthesize from sugars.Microbial cell factories · 2023Article
- Toolbox for the structure-guided evolution of ferulic acid decarboxylase (FDC).Scientific reports · 2022Article
- Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.Frontiers in bioengineering and biotechnology · 2020Review
- Comparative Genomic Analysis of Capsule-Producing Black YeastsFrontiers in microbiology · 2020Article
- Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose.Microbial cell factories · 2019Article
- Enhanced production of styrene by engineered Escherichia coli and in situ product recovery (ISPR) with an organic solvent.Microbial cell factories · 2019Article
- Exploring the substrate scope of ferulic acid decarboxylase (FDC1) from Saccharomyces cerevisiae.Scientific reports · 2019Article
- Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases.ChemCatChem · 2018Article
- An Engineered Aro1 Protein Degradation Approach for IncreasedApplied and environmental microbiology · 2018Article
- Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future Strain Construction Strategies.Frontiers in bioengineering and biotechnology · 2018Article
- Selection of PofFrontiers in microbiology · 2018Article
- Requirement of a Functional Flavin Mononucleotide Prenyltransferase for the Activity of a Bacterial Decarboxylase in a Heterologous Muconic Acid Pathway in Saccharomyces cerevisiae.Applied and environmental microbiology · 2017Article
- Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase.FEMS yeast research · 2017Article
- Systematic development of biomass overproducingSynthetic and systems biotechnology · 2016Article
- Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine.Nature communications · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStyrene is an important building-block petrochemical and monomer used to produce numerous plastics. Whereas styrene bioproduction by Escherichia coli was previously reported, the long-term potential of this approach will ultimately rely on the use of hosts with improved industrial phenotypes, such as the yeast Saccharomyces cerevisiae.
resultsClassical metabolic evolution was first applied to isolate a mutant capable of phenylalanine over-production to 357 mg/L. Transcription analysis revealed up-regulation of several phenylalanine biosynthesis pathway genes including ARO3, encoding the bottleneck enzyme DAHP synthase. To catalyze the first pathway step, phenylalanine ammonia lyase encoded by PAL2 from A. thaliana was constitutively expressed from a high copy plasmid. The final pathway step, phenylacrylate decarboxylase, was catalyzed by the native FDC1. Expression of FDC1 was naturally induced by trans-cinnamate, the pathway intermediate and its substrate, at levels sufficient for ensuring flux through the pathway. Deletion of ARO10 to eliminate the competing Ehrlich pathway and expression of a feedback-resistant DAHP synthase encoded by ARO4K229L preserved and promoted the endogenous availability precursor phenylalanine, leading to improved pathway flux and styrene production. These systematic improvements allowed styrene titers to ultimately reach 29 mg/L at a glucose yield of 1.44 mg/g, a 60% improvement over the initial strain.
conclusionsThe potential of S. cerevisiae as a host for renewable styrene production has been demonstrated. Significant strain improvements, however, will ultimately be needed to achieve economical production levels.
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