ArticlePloS one2014
Upregulation of leukemia inhibitory factor (LIF) during the early stage of optic nerve regeneration in zebrafish.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Srebf2 mediates successful optic nerve axon regeneration via the mevalonate synthesis pathway.Molecular neurodegeneration · 2025Article
- Leukemia inhibitory factor, a double-edged sword with therapeutic implications in human diseases.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Review
- Clcf1/Crlf1a-mediated signaling is neuroprotective and required for Müller glia proliferation in the light-damaged zebrafish retina.Frontiers in cell and developmental biology · 2023Article
- The killifish visual system as an in vivo model to study brain aging and rejuvenation.NPJ aging and mechanisms of disease · 2021Article
- Review
- An Antagonistic Axon-Dendrite Interplay Enables Efficient Neuronal Repair in the Adult Zebrafish Central Nervous System.Molecular neurobiology · 2019Article
- Prior Exposure to Immunosuppressors Sensitizes Retinal Microglia and Accelerates Optic Nerve Regeneration in Zebrafish.Mediators of inflammation · 2019Article
- Beyond Trophic Factors: Exploiting the Intrinsic Regenerative Properties of Adult Neurons.Frontiers in cellular neuroscience · 2019Review
- Optic nerve regeneration in the mouse is a complex trait modulated by genetic background.Molecular vision · 2018Article
- Neurotrophically Induced Mesenchymal Progenitor Cells Derived from Induced Pluripotent Stem Cells Enhance Neuritogenesis via Neurotrophin and Cytokine Production.Stem cells translational medicine · 2018Article
- Neuroinflammation as Fuel for Axonal Regeneration in the Injured Vertebrate Central Nervous System.Mediators of inflammation · 2017Review
- Stat3 Controls Maturation and Terminal Differentiation in Mouse Hippocampal Neurons.Journal of molecular neuroscience : MN · 2017Article
- The Function of Fish Cytokines.Biology · 2016Review
- Overexpression of the monocyte chemokine CCL2 in dorsal root ganglion neurons causes a conditioning-like increase in neurite outgrowth and does so via a STAT3 dependent mechanism.Experimental neurology · 2016Article
- Active mechanistic target of rapamycin plays an ancillary rather than essential role in zebrafish CNS axon regeneration.Frontiers in cellular neuroscience · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fish retinal ganglion cells (RGCs) can regenerate their axons after optic nerve injury, whereas mammalian RGCs normally fail to do so. Interleukin 6 (IL-6)-type cytokines are involved in cell differentiation, proliferation, survival, and axon regrowth; thus, they may play a role in the regeneration of zebrafish RGCs after injury. In this study, we assessed the expression of IL-6-type cytokines and found that one of them, leukemia inhibitory factor (LIF), is upregulated in zebrafish RGCs at 3 days post-injury (dpi). We then demonstrated the activation of signal transducer and activator of transcription 3 (STAT3), a downstream target of LIF, at 3-5 dpi. To determine the function of LIF, we performed a LIF knockdown experiment using LIF-specific antisense morpholino oligonucleotides (LIF MOs). LIF MOs, which were introduced into zebrafish RGCs via a severed optic nerve, reduced the expression of LIF and abrogated the activation of STAT3 in RGCs after injury. These results suggest that upregulated LIF drives Janus kinase (Jak)/STAT3 signaling in zebrafish RGCs after nerve injury. In addition, the LIF knockdown impaired axon sprouting in retinal explant culture in vitro; reduced the expression of a regeneration-associated molecule, growth-associated protein 43 (GAP-43); and delayed functional recovery after optic nerve injury in vivo. In this study, we comprehensively demonstrate the beneficial role of LIF in optic nerve regeneration and functional recovery in adult zebrafish.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.