ArticleMicrobial cell factories2014
Direct production of itaconic acid from liquefied corn starch by genetically engineered Aspergillus terreus.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 52 citations in OpenAlex.
- Evaluating the Potential of Co-supplementation of Zinc and Ferrous Iron Ion for Itaconic Acid Fermentation of Aspergillus terreus.Applied biochemistry and biotechnology · 2025Article
- Metabolic Engineering of Filamentous FungusJournal of agricultural and food chemistry · 2025Article
- Establishing an itaconic acid production process with Ustilago species on the low-cost substrate starch.FEMS yeast research · 2024Article
- Microbial cell factories based on filamentous bacteria, yeasts, and fungi.Microbial cell factories · 2023Review
- Recent Advances in Bio-Based Additive Flame Retardants for Thermosetting Resins.International journal of environmental research and public health · 2022Article
- Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers.Polymers · 2019Review
- Itaconic acid production in microorganisms.Biotechnology letters · 2018Review
- World market and biotechnological production of itaconic acid.3 Biotech · 2018Review
- Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical.Journal of industrial microbiology & biotechnology · 2017Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundItaconic acid is on the DOE (Department of Energy) top 12 list of biotechnologically produced building block chemicals and is produced commercially by Aspergillus terreus. However, the production cost of itaconic acid is too high to be economically competitive with the petrochemical-based products. Itaconic acid is generally produced from raw corn starch, including three steps: enzymatic hydrolysis of corn starch into a glucose-rich syrup by α-amylase and glucoamylase, fermentation, and recovery of itaconic acid. The whole process is very time-consuming and energy-intensive.
resultsIn order to reduce the production cost, saccharification and fermentation were integrated into one step through overexpressing the glucoamylase gene in A. terreus under the control of the native PcitA promoter. The transformant XH61-5 produced higher itaconate titer from liquefied starch than WT. To further increase the titer by enhancing the secretion capacity of overexpressed glucoamylase, a stronger signal peptide was selected based on the major secreted protein ATEG_02176 (an acid phosphatase precursor) by A. terreus under the itaconate production conditions. Under the control of the stronger signal peptide, the transformant XH86-8 showed higher itaconate production level than XH61-5 from liquefied starch. The itaconate titer was further enhanced through a two-step process involving the vegetative and production phase, and the transformant XH86-8 produced comparable itaconate titer from liquefied starch to current one (~80 g/L) from saccharified starch hydrolysates in industry. The effects of the new signal peptide and the two-step process on itaconate production were investigated and discussed.
conclusionsItaconic acid could be efficiently produced from liquefied corn starch by overexpressing the glucoamylase gene in A. terreus, which will be helpful for constructing a highly efficient microbial cell factory for itaconate production and for further lowering the production cost of itaconic acid.
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