ArticleBioMed research international2014
Characterization of human vaginal mucosa cells for autologous in vitro cultured vaginal tissue transplantation in patients with MRKH syndrome.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Methylglyoxal Induced Modifications to Stabilize Therapeutic Proteins: A Review.The protein journal · 2024Review
- Isolation of Vaginal Epithelial Cells: In Preparation of Autologous Vaginal Tissue Lining for Congenital Absence of Vagina Patients.International journal of molecular sciences · 2023Article
- Developing biomedical engineering technologies for reproductive medicine.Smart medicine · 2022Review
- The Embryological Landscape of Mayer-Rokitansky-Kuster-Hauser Syndrome: Genetics and Environmental Factors.The Yale journal of biology and medicine · 2021Review
- Genitourinary Tissue Engineering: Reconstruction and Research Models.Bioengineering (Basel, Switzerland) · 2021Review
- Article
- Protein-protein interaction network analysis applied to DNA copy number profiling suggests new perspectives on the aetiology of Mayer-Rokitansky-Küster-Hauser syndrome.Scientific reports · 2021Article
- A stereological study of 3D printed tissues engineered from rat vaginas.Annals of translational medicine · 2020Article
- The Endometrial Transcription Landscape of MRKH Syndrome.Frontiers in cell and developmental biology · 2020Article
- Multidisciplinary approaches to stimulate wound healing.Annals of the New York Academy of Sciences · 2016Review
- Mayer-Rokitansky-Kuster-Hauser syndrome: a review.International journal of women's health · 2015Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mayer-Rokitansky-Küster-Hauser (MRKH) is a rare syndrome characterized by congenital aplasia of the uterus and vagina. The most common procedure used for surgical reconstruction of the neovagina is the McIndoe vaginoplasty, which consists in creation of a vaginal canal covered with a full-thickness skin graft. Here we characterized the autologous in vitro cultured vaginal tissue proposed as alternative material in our developed modified McIndoe vaginoplasty in order to underlie its importance in autologous total vaginal replacement. To this aim human vaginal mucosa cells (HVMs) were isolated from vaginal mucosa of patients affected by MRKH syndrome and characterized with respect to growth kinetics, morphology, PAS staining, and expression of specific epithelial markers by immunofluorescence, Western blot, and qRT-PCR analyses. The presence of specific epithelial markers along with the morphology and the presence of mucified cells demonstrated the epithelial nature of HMVs, important for an efficient epithelialization of the neovagina walls and for creating a functional vaginal cavity. Moreover, these cells presented characteristics of effective proliferation as demonstrated by growth kinetics assay. Therefore, the autologous in vitro cultured vaginal tissue might represent a highly promising and valid material for McIndoe vaginoplasty.
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