ArticleInternational journal of ophthalmology2014
A comparison of three methods of decellularization of pig corneas to reduce immunogenicity.
Article in International journal of ophthalmology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- dECM bioinks for 3D bioprinted tumor models: Advances, challenges, and drug screening.iScience · 2026Review
- Corneal Endothelium Regeneration with Decellularized Porcine Corneal Extracellular Matrix Scaffolds.Tissue engineering and regenerative medicine · 2025Article
- Development of stromal corneal grafts using a novel decellularization method with sodium cocoyl glutamate onAnnals of translational medicine · 2025Article
- Genetically modified pigs with α1,3-galactosyltransferase knockout and beyond: a comprehensive review of xenotransplantation strategies.Frontiers in immunology · 2025Review
- Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature.Journal of ophthalmology · 2024Review
- Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal Defects.Organogenesis · 2023Article
- The effect of decellularization protocols on characterizations of thermoresponsive and light-curable corneal extracellular matrix hydrogels.Scientific reports · 2023Article
- Biomimetic vascular tissue engineering by decellularized scaffold and concurrent cyclic tensile and shear stresses.Journal of materials science. Materials in medicine · 2023Article
- Corneal endothelial transplantation from bench to bedside: A review of animal models and their translational value for therapeutic development.Experimental eye research · 2022Review
- Lamellar Keratoplasty Using Acellular Bioengineering Cornea (BioCorneaVetAnimals : an open access journal from MDPI · 2022Article
- Corneal xenotransplantation: Where are we standing?Progress in retinal and eye research · 2021Review
- Article
- Comparison of porcine corneal decellularization methods and importance of preserving corneal limbus through decellularization.PloS one · 2021Article
- Article
- Decellularization methods for developing porcine corneal xenografts and future perspectives.Xenotransplantation · 2019Review
- Significantly Accelerated Wound Healing of Full-Thickness Skin Using a Novel Composite Gel of Porcine Acellular Dermal Matrix and Human Peripheral Blood Cells.Cell transplantation · 2017Article
- Decellularization of human stromal refractive lenticules for corneal tissue engineering.Scientific reports · 2016Article
- Use of Fish Scale-Derived BioCornea to Seal Full-Thickness Corneal Perforations in Pig Models.PloS one · 2015Article
- Decellularization for the retention of tissue niches.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
aimTo investigate whether decellularization using different techniques can reduce immunogenicity of the cornea, and to explore the decellularized cornea as a scaffold for cultured corneal endothelial cells (CECs). Transplantation of decellularized porcine corneas increases graft transparency and survival for longer periods compared with fresh grafts.
methodsSix-month-old wild-type pig corneas were cut into 100-200 µm thickness, and then decellularized by three different methods: 1) 0.1% sodium dodecyl sulfate (SDS); 2) hypoxic nitrogen (N2); and 3) hypertonic NaCl. Thickness and transparency were assessed visually. Fresh and decellularized corneas were stained with hematoxylin/eosin (H&E), and for the presence of galactose-α1,3-galactose (Gal) and N-glycolylneuraminic acid (NeuGc, a nonGal antigen). Also, a human IgM/IgG binding assay was performed. Cultured porcine CECs were seeded on the surface of the decellularized cornea and examined after H&E staining.
resultsAll three methods of decellularization reduced the number of keratocytes in the stromal tissue by >80% while the collagen structure remained preserved. No remaining nuclei stained positive for Gal or NeuGc, and expression of these oligosaccharides on collagen was also greatly decreased compared to expression on fresh corneas. Human IgM/IgG binding to decellularized corneal tissue was considerably reduced compared to fresh corneal tissue. The cultured CECs formed a confluent monolayer on the surface of decellularized tissue.
conclusionThough incomplete, the significant reduction in the cellular component of the decellularized cornea should be associated with a significantly reduced in vivo immune response compared to fresh corneas.
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Registered trials
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