Evidence map›Paper›PMID 25161314›Full record

ArticleRNA (New York, N.Y.)2014

Structure of RNA 3'-phosphate cyclase bound to substrate RNA.

Kevin K Desai, Craig A Bingman, Chin L Cheng, George N Phillips, Ronald T Raines

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Kevin K DesaiDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Craig A BingmanDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Chin L ChengDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
George N PhillipsDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA BioSciences at Rice and Department of Chemistry, Rice University, Houston, Texas 77005, USA.
Ronald T RainesDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA rtraines@wisc.edu.
University of Wisconsin–Madison · US

Funding

RIBONUCLEASES IN CANCER THERAPYR01CA073808 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI RAINES, RONALD T · 1997 to 2024
$7.3M
Enzyme Discovery for Natural Product BiosynthesisU01GM098248 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PHILLIPS, GEORGE NEAL · 2011 to 2015
$2.8M
Characterization of a Non-Spliceosomal mRNA LigaseF32GM100681 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI DESAI, KEVIN K · 2012 to 2014
$156k
NCI NIH HHS R01 CA073808NCI NIH HHS Y1-CO-1020NIGMS NIH HHS F32 GM100681NIGMS NIH HHS U01 GM098248NIGMS NIH HHS Y1-GM-1104
6 · The paper itself

Abstract

RNA 3'-phosphate cyclase (RtcA) catalyzes the ATP-dependent cyclization of a 3'-phosphate to form a 2',3'-cyclic phosphate at RNA termini. Cyclization proceeds through RtcA-AMP and RNA(3')pp(5')A covalent intermediates, which are analogous to intermediates formed during catalysis by the tRNA ligase RtcB. Here we present a crystal structure of Pyrococcus horikoshii RtcA in complex with a 3'-phosphate terminated RNA and adenosine in the AMP-binding pocket. Our data reveal that RtcA recognizes substrate RNA by ensuring that the terminal 3'-phosphate makes a large contribution to RNA binding. Furthermore, the RNA 3'-phosphate is poised for in-line attack on the P-N bond that links the phosphorous atom of AMP to N(ε) of His307. Thus, we provide the first insights into RNA 3'-phosphate termini recognition and the mechanism of 3'-phosphate activation by an Rtc enzyme.

Indexed as

Adenosine MonophosphateCatalysisCrystallography, X-RayLigasesModels, MolecularMutagenesis, Site-DirectedMutationProtein BindingProtein ConformationPyrococcus horikoshiiRNAAdenosine MonophosphateLigasesRNARNA 3'-terminal phosphate cyclase2′,3′-cyclic phosphate terminiRNA 3′-phosphate terminiRtcA

Identifiers

PMID25161314
PMCPMC4174438
OpenAlexW2126512827

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.