ArticleParasites & vectors2014
Molecular characterization of flaviviruses from field-collected mosquitoes in northwestern Italy, 2011-2012.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Pooled it
- Complete coding sequence of an Aedes flavivirus strain isolated fromMicrobiology resource announcements · 2024Article
- Entomo-virological surveillance of Flavivirus in mosquitoes in Yucatan State, Mexico.Revista do Instituto de Medicina Tropical de Sao Paulo · 2024Article
- Leveraging insect-specific viruses to elucidate mosquito population structure and dynamics.PLoS pathogens · 2023Review
- A tangled threesome: understanding arbovirus infection inFrontiers in microbiology · 2023Review
- Arbovirus Screening in Mosquitoes in Emilia-Romagna (Italy, 2021) and Isolation of Tahyna Virus.Microbiology spectrum · 2022Article
- Review
- Development of genus-specific universal primers for the detection of flaviviruses.Virology journal · 2021Article
- Surveillance of Mosquitoes and Selected Arthropod-Borne Viruses in the Context of Milan EXPO 2015.International journal of environmental research and public health · 2016Article
- West Nile Virus Circulation in Mosquitoes in Greece (2010-2013).BioMed research international · 2016Article
- Insect-specific viruses and their potential impact on arbovirus transmission.Current opinion in virology · 2015Review
- Arbovirus-mosquito interactions: RNAi pathway.Current opinion in virology · 2015Review
- Ecuador Paraiso Escondido Virus, a New Flavivirus Isolated from New World Sand Flies in Ecuador, Is the First Representative of a Novel Clade in the Genus Flavivirus.Journal of virology · 2015Article
- Article
- Development of a Real-Time Cell Analysing (RTCA) method as a fast and accurate screen for the selection of chikungunya virus replication inhibitors.Parasites & vectors · 2015Article
- Genome sequence of the Asian Tiger mosquito, Aedes albopictus, reveals insights into its biology, genetics, and evolution.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mosquito surveillance for prevention and control of emerging mosquito-borne diseases in Portugal - 2008-2014.International journal of environmental research and public health · 2014Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genus Flavivirus comprises several mosquito-borne species, including the zoonotic pathogens West Nile and Usutu virus, circulating in animals and humans in Italy since 1998. Due to its ecological and geographical features, Piedmont is considered a risk area for flavivirus transmission. Here we report the results of a flavivirus survey (detection and genetic characterization) of mosquitoes collected in Piedmont in 2012 and the genetic characterization of three strains detected in 2011.
methodsPools of 1-203 mosquitoes, upon RNA extraction with TRIzol, were screened by a PCR assay for a 263 bp fragment of the Flavivirus NS5 gene. All positive samples were tested with a specific PCR for the E protein gene of Usutu virus and a generic Flavivirus RT-nested-PCR for a larger tract of the NS5 gene before sequencing. Phylogenetic trees were built with both NS5 fragments of representative Flavivirus species. DNA extracts of part of the positive pools were tested to detect sequences integrated in the host genome.
resultsThirty-four mosquito pools resulted positive for flaviviruses, and twenty-five flavivirus sequences underwent phylogenetic analysis for the short NS5 fragment. Among the 19 sequences correlating with the insect-specific flavivirus group, ten samples, retrieved from Aedes albopictus, clustered within Aedes flavivirus, while the other nine aggregated in a separate clade composed of strains from various mosquito species (mainly Aedes vexans) from Piedmont and the Czech Republic. Six out of these nine also presented a DNA form of the sequence. The remaining sequences belonged to the mosquito-borne group: four, all from Culex pipiens, correlated to Italian Usutu virus strains, whereas two, from Ochlerotatus caspius, were highly similar to Marisma mosquito virus (MMV).
conclusionsOur findings confirm the circulation of Usutu virus and of the potentially zoonotic Marisma mosquito virus in Piedmont. This is the first detection of Aedes flavivirus in Piedmont. Finally, further evidence for the integration of Flavivirus nucleic acid into the host genome has been shown. These results underline the importance of continuing intense mosquito-based surveillance in Piedmont, supported by a mosquito control program in areas at high risk for human exposure.
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