ArticleThe Journal of antibiotics2015
Small colony variants have a major role in stability and persistence of Staphylococcus aureus biofilms.
Article in The Journal of antibiotics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
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- Significant Changes in Cytoplasmic Amino Acid Composition Occur in the Transition between Mid-Exponential and Stationary Phases of Growth ofMicroorganisms · 2023Article
- Recent Advances in the Control of Clinically Important Biofilms.International journal of molecular sciences · 2022Review
- How Good are Bacteriophages as an Alternative Therapy to Mitigate Biofilms of Nosocomial Infections.Infection and drug resistance · 2022Review
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- An effective weapon against biofilm consortia and small colony variants of MRSA.Iranian journal of basic medical sciences · 2020Article
- Selected Metabolites Profiling ofFrontiers in microbiology · 2020Article
- Novel Research Models forFrontiers in microbiology · 2020Review
- Antimicrobial Susceptibility Testing of Antimicrobial Peptides to Better Predict Efficacy.Frontiers in cellular and infection microbiology · 2020Review
- Hypericin enhancesActa pharmaceutica Sinica. B · 2019Article
- Genomic and transcriptomic characterization of Pseudomonas aeruginosa small colony variants derived from a chronic infection model.Microbial genomics · 2019Article
- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study onIranian journal of basic medical sciences · 2018Article
- Posttranslational modification of a histone-like protein regulates phenotypic resistance to isoniazid in mycobacteria.Science advances · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study was conducted to investigate the significance of small colony variants (SCVs) in biofilm life cycle of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA). All of these MRSA and MSSA isolates were recovered from different food commodities. Molecular typing showed that 21 MRSA isolates carry SCCmecA type IV and belong to agr type II. Out of 15 MSSA isolates, 7 were found to carry agr type II, 5 agr type I and 2 agr type III. All of the MRSA isolates studied adopted biofilm mode of growth after exposure to sublethal doses of oxacillin. MSSA isolates, on the other hand, were biofilm producers by nature, that is, without exposure to any stress. The biomass of the biofilm reaches its maximum thickness after 48 h of incubation at 35 °C. It was noticed that biofilm population consists of wild type and SCVs. Moreover, the number of SCVs increases with the age of biofilm. The SCVs of MRSA were unable to readopt biofilm mode of growth independently, irrespective of the presence or absence of oxacillin. The SCVs of MSSA, on the other hand, quickly revert to normal life just after a single subculture and show biofilm formation without any stress. Molecular studies showed a parallel reduction in the expression of the genes icaA, sigβ and sarA, and also in the extracellular matrix production in SCVs of MRSA. This might be due to oxacillin as it seems to be a stress factor responsible for induction of biofilm formation in MRSA isolates. Contrary to the wild type, SCVs are metabolically inactive and do not respond to oxacillin, which is only active against the growing cells. Therefore, stress-responsive genes, that is, sigβ and sarA, are not induced. Conversely, MSSA isolates are natural biofilm producers without induction through any known factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.