ArticleAngewandte Chemie (International ed. in English)2014
Pyrene-based quantitative detection of the 5-formylcytosine loci symmetry in the CpG duplex content during TET-dependent demethylation.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Spectroscopic and in vitro Investigations of FeChembiochem : a European journal of chemical biology · 2022Review
- Evolved DNA Duplex Readers for Strand-Asymmetrically Modified 5-Hydroxymethylcytosine/5-Methylcytosine CpG Dyads.Journal of the American Chemical Society · 2022Article
- Quantification and mapping of DNA modifications.RSC chemical biology · 2021Review
- Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency.Scientific reports · 2020Article
- Direct observation and analysis of TET-mediated oxidation processes in a DNA origami nanochip.Nucleic acids research · 2020Article
- Complete Profiling of Methyl-CpG-Binding Domains for Combinations of Cytosine Modifications at CpG Dinucleotides Reveals Differential Read-out in Normal and Rett-Associated States.Scientific reports · 2020Article
- Article
- Gene specific-loci quantitative and single-base resolution analysis of 5-formylcytosine by compound-mediated polymerase chain reaction.Chemical science · 2018Article
- Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA.Chemical science · 2017Article
- Application of Ammonium Persulfate for Selective Oxidation of Guanines for Nucleic Acid Sequencing.Molecules (Basel, Switzerland) · 2017Article
- Enrichment and fluorogenic labelling of 5-formyluracil in DNA.Chemical science · 2017Article
- The expanding scope and impact of epigenetic cytosine modifications.Current opinion in chemical biology · 2016Review
- 5-Formylcytosine alters the structure of the DNA double helix.Nature structural & molecular biology · 2015Article
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Authors and funding
8 authors.
Funding
Abstract
Methylcytosine (5mC) is mostly symmetrically distributed in CpG sites. Ten-eleven-translocation (TET) proteins are the key enzymes involved in active DNA demethylation through stepwise oxidation of 5mC. However, oxidation pathways of TET enzymes in the symmetrically methylated CpG context are still elusive. Employing the unique fluorescence properties of pyrene group, we designed and synthesized a sensitive fluorescence-based probe not only to target 5-formylcytosine (5fC) sites, but also to distinguish symmetric from asymmetric 5fC sites in the double stranded DNA context during TET-dependent 5mC oxidation process. Using this novel probe, we revealed dominant levels of symmetric 5fC among total 5fC sites during in vitro TET-dependent 5mC oxidation and novel mechanistic insights into the TET-dependent 5mC oxidation in the mCpG context.
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