ArticleAmerican journal of physiology. Endocrinology and metabolism2014
Fascin 1 is an actin filament-bundling protein that regulates ectoplasmic specialization dynamics in the rat testis.
Article in American journal of physiology. Endocrinology and metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Actin Cytoskeleton Dysregulation Links Testicular and Sperm Dysfunction in Type 1 Diabetes.International journal of molecular sciences · 2026Article
- CAPZA1 deficiency disrupts sperm flagellar structure and motility, potentially involving the p300/SLC7A11 pathway.Frontiers in endocrinology · 2026Article
- Statistical methods to disentangle genetic effects influencing infertility and early fetal viability with a genome-wide application.PLoS genetics · 2025Article
- Identification of hub genes associated with spermatogenesis by bioinformatics analysis.Scientific reports · 2023Article
- Fascin-1: Updated biological functions and therapeutic implications in cancer biology.BBA advances · 2022Review
- Spermiation: Insights from Studies on the Adjudin Model.Advances in experimental medicine and biology · 2021Review
- Roles of Fascin mRNA expression in colorectal cancer: Meta-analysis and bioinformatics analysis.Molecular and clinical oncology · 2020Article
- High Expression Levels of Fascin-1 Protein in Human Gliomas and its Clinical Relevance.Open medicine (Warsaw, Poland) · 2018Article
- Drebrin and Spermatogenesis.Advances in experimental medicine and biology · 2017Article
- Melatonin Represses Metastasis in Her2-Postive Human Breast Cancer Cells by Suppressing RSK2 Expression.Molecular cancer research : MCR · 2016Article
- Is toxicant-induced Sertoli cell injury in vitro a useful model to study molecular mechanisms in spermatogenesis?Seminars in cell & developmental biology · 2016Review
- Review
- Regulation of blood-testis barrier by actin binding proteins and protein kinases.Reproduction (Cambridge, England) · 2016Review
- The Mammalian Blood-Testis Barrier: Its Biology and Regulation.Endocrine reviews · 2015Review
- RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.PLoS genetics · 2015Article
- Actin binding proteins in blood-testis barrier function.Current opinion in endocrinology, diabetes, and obesity · 2015Review
- Sertoli cells are the target of environmental toxicants in the testis - a mechanistic and therapeutic insight.Expert opinion on therapeutic targets · 2015Review
- Fascin - An actin binding and bundling protein in the testis and its role in ectoplasmic specialization dynamics.SpermatogenesisArticle
- Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro.Asian journal of andrologyArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
In the testis, spermatids are polarized cells, with their heads pointing toward the basement membrane during maturation. This polarity is crucial to pack the maximal number of spermatids in the seminiferous epithelium so that millions of sperms can be produced daily. A loss of spermatid polarity is detected after rodents are exposed to toxicants (e.g., cadmium) or nonhormonal male contraceptives (e.g., adjudin), which is associated with a disruption on the expression and/or localization of polarity proteins. In the rat testis, fascin 1, an actin-bundling protein found in mammalian cells, was expressed by Sertoli and germ cells. Fascin 1 was a component of the ectoplasmic specialization (ES), a testis-specific anchoring junction known to confer spermatid adhesion and polarity. Its expression in the seminiferous epithelium was stage specific. Fascin 1 was localized to the basal ES at the Sertoli cell-cell interface of the blood-testis barrier in all stages of the epithelial cycle, except it diminished considerably at late stage VIII. Fascin 1 was highly expressed at the apical ES at stage VII-early stage VIII and restricted to the step 19 spermatids. Its knockdown by RNAi that silenced fascin 1 by ~70% in Sertoli cells cultured in vitro was found to perturb the tight junction-permeability barrier via a disruption of F-actin organization. Knockdown of fascin 1 in vivo by ~60-70% induced defects in spermatid polarity, which was mediated by a mislocalization and/or downregulation of actin-bundling proteins Eps8 and palladin, thereby impeding F-actin organization and disrupting spermatid polarity. In summary, these findings provide insightful information on spermatid polarity regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.