ArticleMicrobial cell factories2014
Exploiting Issatchenkia orientalis SD108 for succinic acid production.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 113 citations in OpenAlex.
- Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects.Synthetic and systems biotechnology · 2027Review
- Unlock the Potential of Pichia in Coffee: Knowledge Transfer From Fermented Beverages.Comprehensive reviews in food science and food safety · 2026Review
- Engineering a robust cell-surface display platform in the multi-stress-tolerant yeast Issatchenkia orientalis.Applied microbiology and biotechnology · 2026Article
- Temporal and spatial shifts in the foliar fungal endophyte community of Norway spruce (Picea abies) over 150 years in Finland.Environmental microbiome · 2026Article
- Bio-based oxalic acid production in Issatchenkia orientalis enables sustainable rare earth recovery.Nature communications · 2026Article
- High yield production of 3-hydroxypropionic acid using Issatchenkia orientalis.Nature communications · 2026Article
- Draft genome sequence of a kombucha isolate ofMicrobiology resource announcements · 2025Article
- Longitudinal alterations of gut mycobiota during 2 years after COVID-19 and its correlation with pulmonary sequela.Microbiology spectrum · 2025Article
- Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration.FEMS yeast research · 2025Review
- Aerobic glycolysis comes with an enzyme cost but robustness gain.Nature chemical biology · 2024Article
- Mitochondrial ATP generation is more proteome efficient than glycolysis.Nature chemical biology · 2024Article
- Genomic factors shape carbon and nitrogen metabolic niche breadth across Saccharomycotina yeasts.Science (New York, N.Y.) · 2024Article
- Kinetic modeling and optimization of ethanol fermentation by the marine yeast Wickerhamomyces subpelliculosus ZE75.World journal of microbiology & biotechnology · 2024Article
- Selected Ion Monitoring for Orbitrap-Based Metabolomics.Metabolites · 2024Article
- A transcriptomic atlas of acute stress response to low pH in multipleMicrobiology spectrum · 2024Article
- Mitochondrial membrane transporters as attractive targets for the fermentative production of succinic acid from glycerol in Saccharomyces cerevisiae.FEMS yeast research · 2024Article
- Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica.Nature communications · 2023Article
- An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis.Nature communications · 2023Article
- Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid.Nature communications · 2023Article
- Metabolic engineering of low-pH-tolerant non-model yeast,Metabolic engineering communications · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable.
resultsHere we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 and Classic Distiller's Turbo yeast. In order to explore SD108 as a potential platform organism for organic acid production, we determined its draft genome sequence and use the sequencing information to guide pathway design. As proof of concept, an engineered four-gene expression cassette related to the reductive TCA cycle was assembled and integrated into the genome of a uracil auxotroph of SD108. The resulting strain was able to produce succinic acid with a titer of 11.63 g/L, yield of 0.12 g/g, and productivity of 0.11 g/L · h in batch cultures using shake flasks.
conclusionsThe high tolerance of I. orientalis SD108 towards multiple important organic acids makes it a highly attractive organism as a platform host for producing this group of compounds as it will reduce production cost, facilitate downstream processing, and serve as a host for construction of production strains with both pH and specific anion tolerance.
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