ArticleTraffic (Copenhagen, Denmark)2014
Quantitative analysis of membrane trafficking in regulation of Cdc42 polarity.
Article in Traffic (Copenhagen, Denmark), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- The origin of septin ring size control in budding yeast.The EMBO journal · 2025Article
- Cdc42 mobility and membrane flows regulate fission yeast cell shape and survival.Nature communications · 2024Article
- Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.The Journal of cell biology · 2024Article
- Article
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- The Arp2/3 complex promotes periodic removal of Pak1-mediated negative feedback to facilitate anticorrelated Cdc42 oscillations.bioRxiv : the preprint server for biology · 2023Article
- Particle-based simulations reveal two positive feedback loops allow relocation and stabilization of the polarity site during yeast mating.PLoS computational biology · 2023Article
- Conjugated Polymeric Materials in Biological Imaging and Cancer Therapy.Molecules (Basel, Switzerland) · 2023Review
- A novel stochastic simulation approach enables exploration of mechanisms for regulating polarity site movement.PLoS computational biology · 2021Article
- Review
- A Fully Functional ROP Fluorescent Fusion Protein Reveals Roles for This GTPase in Subcellular and Tissue-Level Patterning.The Plant cell · 2020Article
- Regulation of Cdc42 for polarized growth in budding yeast.Microbial cell (Graz, Austria) · 2020Review
- Mechanistic insights into actin-driven polarity site movement in yeast.Molecular biology of the cell · 2020Article
- Extraction of active RhoGTPases by RhoGDI regulates spatiotemporal patterning of RhoGTPases.eLife · 2019Article
- Polarity establishment by Cdc42: Key roles for positive feedback and differential mobility.Small GTPases · 2019Review
- Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.The Journal of cell biology · 2018Article
- Cloning and Differential Expression Analyses of Cdc42 from Sheep.Journal of veterinary research · 2018Article
- Particle-based simulations of polarity establishment reveal stochastic promotion of Turing pattern formation.PLoS computational biology · 2018Article
- LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling.The Journal of cell biology · 2018Article
- Cell Polarity in Yeast.Annual review of cell and developmental biology · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vesicle delivery of Cdc42 has been proposed as an important mechanism for generating and maintaining Cdc42 polarity at the plasma membrane. This mechanism requires the density of Cdc42 on secretory vesicles to be equal to or higher than the plasma membrane polarity cap. Using a novel method to estimate Cdc42 levels on post-Golgi secretory vesicles in intact yeast cells, we: (1) determined that endocytosis plays an important role in Cdc42's association with secretory vesicles (2) found that a GFP-tag placed on the N-terminus of Cdc42 negatively impacts this vesicle association and (3) quantified the surface densities of Cdc42 on post-Golgi vesicles which revealed that the vesicle density of Cdc42 is three times more dilute than that at the polarity cap. This work suggests that the immediate consequence of secretory vesicle fusion with the plasma membrane polarity cap is to dilute the local Cdc42 surface density. This provides strong support for the model in which vesicle trafficking acts to negatively regulate Cdc42 polarity on the cell surface while also providing a means to recycle Cdc42 between the cell surface and internal membrane locations.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.