ArticleJournal of molecular biology2014
DNA recognition by a σ(54) transcriptional activator from Aquifex aeolicus.
Article in Journal of molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Disrupting NtrC function reveals unexpected robustness in a central cell cycle regulatory network.mBio · 2025Article
- Identification of critical amino acids in the DNA binding domain of LuxO: Lessons from a constitutive active LuxO.PloS one · 2024Article
- REC domain stabilizes the active heptamer of σiScience · 2023Article
- Purification and Biochemical Characterization of the DNA Binding Domain of the Nitrogenase Transcriptional Activator NifA from Gluconacetobacter diazotrophicus.The protein journal · 2023Article
- Subtle sequence differences between two interacting σThe FEBS journal · 2022Article
- Crystal structure of the nucleoid-associated protein Fis (PA4853) from Pseudomonas aeruginosa.Acta crystallographica. Section F, Structural biology communications · 2020Article
- The Azoarcus anaerobius 1,3-Dihydroxybenzene (Resorcinol) Anaerobic Degradation Pathway Is Controlled by the Coordinated Activity of Two Enhancer-Binding Proteins.Applied and environmental microbiology · 2017Article
- The EbpA-RpoN Regulatory Pathway of the Pathogen Leptospira interrogans Is Essential for Survival in the Environment.Applied and environmental microbiology · 2017Article
- Kinetics of transcription initiation directed by multiple cis-regulatory elements on the glnAp2 promoter.Nucleic acids research · 2016Article
- Structure, function, and tethering of DNA-binding domains in σ⁵⁴ transcriptional activators.Biopolymers · 2013Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Transcription initiation by bacterial σ(54)-polymerase requires the action of a transcriptional activator protein. Activators bind sequence-specifically upstream of the transcription initiation site via a DNA-binding domain (DBD). The structurally characterized DBDs from activators all belong to the Fis (factor for inversion stimulation) family of helix-turn-helix DNA-binding proteins. We report here structures of the free and DNA-bound forms of the DBD of NtrC4 (4DBD) from Aquifex aeolicus, a member of the NtrC family of σ(54) activators. Two NtrC4-binding sites were identified upstream (-145 and -85bp) from the start of the lpxC gene, which is responsible for the first committed step in lipid A biosynthesis. This is the first experimental evidence for σ(54) regulation in lpxC expression. 4DBD was crystallized both without DNA and in complex with the -145-binding site. The structures, together with biochemical data, indicate that NtrC4 binds to DNA in a manner that is similar to that of its close homolog, Fis. The greater sequence specificity for the binding of 4DBD relative to Fis seems to arise from a larger number of base-specific contacts contributing to affinity than for Fis.
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