ArticlePloS one2014
Nuclear receptor subfamily 2 group F member 1a (nr2f1a) is required for vascular development in zebrafish.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 23 citations in OpenAlex.
- Disruption of Swell1/VRAC function impairs initial hemodynamics and activates compensatory leukotriene signaling in zebrafish circulation development.Frontiers in cell and developmental biology · 2025Article
- Involvement of nr2f genes in brain regionalization and eye development during early zebrafish development.Development, growth & differentiation · 2024Article
- Transcription factor induction of vascular blood stem cell niches in vivo.Developmental cell · 2023Article
- Nitrobenzoate-Derived Compound X8 Impairs Vascular Development in Zebrafish.International journal of molecular sciences · 2022Article
- Identification of Novel Vascular Genes Downstream of Islet2 and Nr2f1b Transcription Factors.Biomedicines · 2022Article
- Article
- Identification of ACTA2 as a Key Contributor to Venous Malformation.Frontiers in cell and developmental biology · 2021Article
- Disruption of Nuclear Receptor Signaling Alters Triphenyl Phosphate-Induced Cardiotoxicity in Zebrafish Embryos.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
- Nr2f1a balances atrial chamber and atrioventricular canal size via BMP signaling-independent and -dependent mechanisms.Developmental biology · 2018Article
- Transgenic Expression of A Venous Malformation Related Mutation,International journal of medical sciences · 2018Article
- Coral-Derived Natural Marine Compound GB9 Impairs Vascular Development in Zebrafish.International journal of molecular sciences · 2017Article
- Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development.Scientific reports · 2017Article
- Ftr82 Is Critical for Vascular Patterning during Zebrafish Development.International journal of molecular sciences · 2017Article
- Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development.Journal of biomedical science · 2015Article
- Development of hair cells in inner ear is associated with expression and promoter methylation of Notch-1 in postnatal mice.International journal of clinical and experimental medicine · 2015Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic regulators and signaling pathways are important for the formation of blood vessels. Transcription factors controlling vein identity, intersegmental vessels (ISV) growth and caudal vein plexus (CVP) formation in zebrafish are little understood as yet. Here, we show the importance of the nuclear receptor subfamily member 1A (nr2f1a) in zebrafish vascular development. Amino acid sequence alignment and phylogenetic analysis of nr2f1a is highly conserved among the vertebrates. Our in situ hybridization results showed nr2f1a mRNA is expressed in the lateral plate mesoderm at 18 somite stage and in vessels at 24-30 hpf, suggesting its roles in vasculization. Consistent with this morpholino-based knockdown of nr2fla impaired ISV growth and failed to develop fenestrated vascular structure in CVP, suggesting that nr2f1a has important roles in controlling ISV and CVP growth. Consequently, nr2f1a morphants showed pericardial edema and circulation defects. We further demonstrated reduced ISV cells and decreased CVP endothelial cells sprouting in nr2f1a morphants, indicating the growth impairment of ISV and CVP is due to a decrease of cell proliferation and migration, but not results from cell death in endothelial cells after morpholino knockdown. To test molecular mechanisms and signals that are associated with nr2f1a, we examined the expression of vascular markers. We found that a loss of nr2f1a results in a decreased expression of vein/ISV specific markers, flt4, mrc1, vascular markers stabilin and ephrinb2. This indicates the regulatory role of nr2f1a in controlling vascular development. We further showed that nr2f1a likely interact with Notch signaling by examining nr2f1a expression in rbpsuh morphants and DAPT-treatment embryos. Together, we show nr2f1a plays a critical role for vascular development in zebrafish.
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