ArticlePloS one2014
Transcriptome wide identification and validation of calcium sensor gene family in the developing spikes of finger millet genotypes for elucidating its role in grain calcium accumulation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- Finger Millet: A Genetic Resource for Calcium and Iron Biofortification in Staple Crops.Molecular nutrition & food research · 2026Review
- Review
- Biotechnological approaches to reduce the phytic acid content in millets to improve nutritional quality.Planta · 2024Review
- Biotechnological studies towards improvement of finger millet using multi-omics approaches.Functional & integrative genomics · 2024Review
- Review
- Review
- Genetic and genomic interventions in crop biofortification: Examples in millets.Frontiers in plant science · 2023Review
- Comprehensive identification and analysis of circRNAs during hickory (Frontiers in plant science · 2022Article
- Genetic determinants of micronutrient traits in graminaceous crops to combat hidden hunger.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Genome-wide analysis and expression profiles of PdeMYB transcription factors in colored-leaf poplar (Populus deltoids).BMC plant biology · 2021Article
- MainstreamingBiodiversity and conservation · 2021Review
- Microbial-assisted and genomic-assisted breeding: a two way approach for the improvement of nutritional quality traits in agricultural crops.3 Biotech · 2020Review
- Insights on Calcium-Dependent Protein Kinases (CPKs) Signaling for Abiotic Stress Tolerance in Plants.International journal of molecular sciences · 2019Review
- Review
- Genome-wide Identification andThe plant genome · 2019Article
- Article
- Finger Millet [Frontiers in plant science · 2018Review
- Molecular characterization of3 Biotech · 2017Article
- Identification and expression analysis under abiotic stress of thePhysiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Harnessing Finger Millet to Combat Calcium Deficiency in Humans: Challenges and Prospects.Frontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn finger millet, calcium is one of the important and abundant mineral elements. The molecular mechanisms involved in calcium accumulation in plants remains poorly understood. Transcriptome sequencing of genetically diverse genotypes of finger millet differing in grain calcium content will help in understanding the trait. PRINCIPAL FINDING: In this study, the transcriptome sequencing of spike tissues of two genotypes of finger millet differing in their grain calcium content, were performed for the first time. Out of 109,218 contigs, 78 contigs in case of GP-1 (Low Ca genotype) and out of 120,130 contigs 76 contigs in case of GP-45 (High Ca genotype), were identified as calcium sensor genes. Through in silico analysis all 82 unique calcium sensor genes were classified into eight calcium sensor gene family viz., CaM & CaMLs, CBLs, CIPKs, CRKs, PEPRKs, CDPKs, CaMKs and CCaMK. Out of 82 genes, 12 were found diverse from the rice orthologs. The differential expression analysis on the basis of FPKM value resulted in 24 genes highly expressed in GP-45 and 11 genes highly expressed in GP-1. Ten of the 35 differentially expressed genes could be assigned to three documented pathways involved mainly in stress responses. Furthermore, validation of selected calcium sensor responder genes was also performed by qPCR, in developing spikes of both genotypes grown on different concentration of exogenous calcium.
conclusionThrough de novo transcriptome data assembly and analysis, we reported the comprehensive identification and functional characterization of calcium sensor gene family. The calcium sensor gene family identified and characterized in this study will facilitate in understanding the molecular basis of calcium accumulation and development of calcium biofortified crops. Moreover, this study also supported that identification and characterization of gene family through Illumina paired-end sequencing is a potential tool for generating the genomic information of gene family in non-model species.
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