ArticleSensors (Basel, Switzerland)2014
An immunosensor based on antibody binding fragments attached to gold nanoparticles for the detection of peptides derived from avian influenza hemagglutinin H5.
Article in Sensors (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 53 citations in OpenAlex.
- Development of Optical Label-Free Biosensor Method in Detection ofSensors (Basel, Switzerland) · 2023Article
- Paper-based electrochemical immunosensor for label-free detection of multiple avian influenza virus antigens using flexible screen-printed carbon nanotube-polydimethylsiloxane electrodes.Scientific reports · 2022Article
- Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2020Review
- Cardiac Troponin I: Ultrasensitive Detection Using Faradaic Electrochemical Impedance.ACS omega · 2018Article
- Applications of Gold Nanoparticles in Non-Optical Biosensors.Nanomaterials (Basel, Switzerland) · 2018Review
- Development of a Label-Free Immunosensor for Clusterin Detection as an Alzheimer's Biomarker.Sensors (Basel, Switzerland) · 2018Article
- Detecting and Predicting Emerging Disease in Poultry With the Implementation of New Technologies and Big Data: A Focus on Avian Influenza Virus.Frontiers in veterinary science · 2018Review
- Fluorescence-Free Biosensor Methods in Detection of Food Pathogens with a Special Focus on Listeria monocytogenes.Biosensors · 2017Review
- Advanced nanotechnologies in avian influenza: Current status and future trends - A review.Analytica chimica acta · 2017Review
- Nanomaterial-based sensors for the detection of biological threat agents.Materials today (Kidlington, England) · 2016Review
- Antibodies and antibody-derived analytical biosensors.Essays in biochemistry · 2016Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper concerns the development of an immunosensor for detection of peptides derived from avian influenza hemagglutinin H5. Its preparation consists of successive gold electrode modification steps: (i) modification with 1,6-hexanedithiol and gold colloidal nanoparticles; (ii) immobilization of antibody-binding fragments (Fab') of anti-hemagglutinin H5 monoclonal antibodies Mab 6-9-1 via S-Au covalent bonds; and (iii) covering the remaining free space on the electrode surfaces with bovine serum albumin. The interactions between Fab' fragments and hemagglutinin (HA) variants have been explored with electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)6](3-/4-) as an electroactive marker. The immunosensor was able to recognize three different His-tagged variants of recombinant hemagglutinin from H5N1 viruses: H1 subunit (17-340 residues) of A/swan/Poland/305-135V08/2006, the long HA (17-530 residues) A/Bar-headed Goose/Qinghai/12/2005 and H1 subunit (1-345 residues) of A/Vietnam/1194/2004. The strongest response has been observed for the long variant with detection limit of 2.2 pg/mL and dynamic range from 4.0 to 20.0 pg/mL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.