ArticleProceedings of the National Academy of Sciences of the United States of America2014
Bacterial formate hydrogenlyase complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
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Who cites it
124 citing papers in PubMed, 265 citations in OpenAlex.
- Transcriptional regulatory network differences between six industrialMetabolic engineering communications · 2026Article
- Genetic dissection of formate hydrogenlyase-2 function: a metalloenzyme involved in bacterial hydrogen production.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- Semiartificial COChemical reviews · 2026Review
- Formate hydrogenlyase is essential for pH homeostasis, maintenance of ATP levels, and COJournal of bacteriology · 2026Article
- Lessons, connections, hypotheses and predictions from protein film electrochemistry.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Review
- Native HNature chemistry · 2026Article
- Illuminating the mechanism: gene expression responses to antimicrobial photodynamic therapy.BMC genomics · 2026Review
- Clostridium beijerinckii displays a soluble [FeFe]-hydrogenase/formate dehydrogenase enzyme complex that links H2 and CO2 metabolism.The Biochemical journal · 2026Article
- Disrupting the mtr-operon in Methanosarcina acetivorans enables methyl-reducing methanogenesis with hydrogen and serine as the alternative electron donors.FEMS microbiology letters · 2026Article
- The Adaptations ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2026Article
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- Sustainable co-production of 1,3-PDO, ethanol and HFrontiers in bioengineering and biotechnology · 2026Article
- Genomics-based insights into the expanded diversity and adaptation strategies of hadal trench anammox bacteria.ISME communications · 2026Article
- Search for the role of a Huc-type [NiFe]-hydrogenase of the soil thermophileFrontiers in microbiology · 2026Article
- Comparative transcriptomic analysis of perfluoroalkyl substances-induced responses of exponential and stationary phase Escherichia coli.BMC genomics · 2025Article
- Microbial Conversion of Glycerol Into 1,3-Propanediol by Fermentation: Review of Fundamentals and Operational Strategies.Microbial biotechnology · 2025Review
- A widespread hydrogenase supports fermentative growth of gut bacteria in healthy people.Nature microbiology · 2025Article
- IL-1β alters the virulence of uropathogenic Escherichia coli.Scientific reports · 2025Article
- Genomics and physiological characterizations of an acidotolerant nitrite-oxidizingApplied and environmental microbiology · 2025Article
- Modeling reveals a metabolic basis of competition amongmSystems · 2025Article
64 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Under anaerobic conditions, Escherichia coli can carry out a mixed-acid fermentation that ultimately produces molecular hydrogen. The enzyme directly responsible for hydrogen production is the membrane-bound formate hydrogenlyase (FHL) complex, which links formate oxidation to proton reduction and has evolutionary links to Complex I, the NADH:quinone oxidoreductase. Although the genetics, maturation, and some biochemistry of FHL are understood, the protein complex has never been isolated in an intact form to allow biochemical analysis. In this work, genetic tools are reported that allow the facile isolation of FHL in a single chromatographic step. The core complex is shown to comprise HycE (a [NiFe] hydrogenase component termed Hyd-3), FdhF (the molybdenum-dependent formate dehydrogenase-H), and three iron-sulfur proteins: HycB, HycF, and HycG. A proportion of this core complex remains associated with HycC and HycD, which are polytopic integral membrane proteins believed to anchor the core complex to the cytoplasmic side of the membrane. As isolated, the FHL complex retains formate hydrogenlyase activity in vitro. Protein film electrochemistry experiments on Hyd-3 demonstrate that it has a unique ability among [NiFe] hydrogenases to catalyze production of H2 even at high partial pressures of H2. Understanding and harnessing the activity of the FHL complex is critical to advancing future biohydrogen research efforts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.