ArticleProceedings of the National Academy of Sciences of the United States of America2014
Three-dimensional cell body shape dictates the onset of traction force generation and growth of focal adhesions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Basigin (CD147) and calpain 4 (CapnS1) are partners in the generation of traction force but not in mechanosensing.The Journal of biological chemistry · 2026Article
- Computational insights on the interplay between electrotaxis and mechanotaxis.PLoS computational biology · 2026Article
- Allometric cell spreading and the geometrical control of focal adhesion collective organization.Biophysical journal · 2025Article
- Morphodynamics of T-lymphocytes: Scanning to spreading.Biophysical journal · 2024Review
- Nucleation of cadherin clusters on cell-cell interfaces.Scientific reports · 2022Article
- Optineurin links Hace1-dependent Rac ubiquitylation to integrin-mediated mechanotransduction to control bacterial invasion and cell division.Nature communications · 2022Article
- Topological Distribution of Wound Stiffness Modulates Wound-Induced Hair Follicle Neogenesis.Pharmaceutics · 2022Article
- Integrins in the Regulation of Mesenchymal Stem Cell Differentiation by Mechanical Signals.Stem cell reviews and reports · 2022Review
- An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Development of Nascent Focal Adhesions in Spreading Cells.Biophysical journal · 2020Review
- Cytoskeletal organization in isolated plant cells under geometry control.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction.Frontiers in bioengineering and biotechnology · 2019Review
- Effect of Cytoskeleton Elasticity on Amoeboid Swimming.Biophysical journal · 2018Article
- Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction.Bioengineering (Basel, Switzerland) · 2017Review
- Biophysical Regulation of Cell Behavior-Cross Talk between Substrate Stiffness and Nanotopography.Engineering (Beijing, China) · 2017Article
- Establishing the Basis for Mechanobiology-Based Physical Therapy Protocols to Potentiate Cellular Healing and Tissue Regeneration.Frontiers in physiology · 2017Review
- Laser-based technique for controlled damage of mesenchymal cell spheroids: a first step in studying reparation in vitro.Biology open · 2016Article
- Desmin Mutation in the C-Terminal Domain Impairs Traction Force Generation in Myoblasts.Biophysical journal · 2016Article
- Cost-benefit analysis of the mechanisms that enable migrating cells to sustain motility upon changes in matrix environments.Journal of the Royal Society, Interface · 2015Article
- Cells as liquid motors: mechanosensitivity emerges from collective dynamics of actomyosin cortex.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell shape affects proliferation and differentiation, which are processes known to depend on integrin-based focal adhesion (FA) signaling. Because shape results from force balance and FAs are mechanosensitive complexes transmitting tension from the cell structure to its mechanical environment, we investigated the interplay between 3D cell shape, traction forces generated through the cell body, and FA growth during early spreading. Combining measurements of cell-scale normal traction forces with FA monitoring, we show that the cell body contact angle controls the onset of force generation and, subsequently, the initiation of FA growth at the leading edge of the lamella. This suggests that, when the cell body switches from convex to concave, tension in the apical cortex is transmitted to the lamella where force-sensitive FAs start to grow. Along this line, increasing the stiffness resisting cell body contraction led to a decrease of the lag time between force generation and FA growth, indicating mechanical continuity of the cell structure and force transmission from the cell body to the leading edge. Remarkably, the overall normal force per unit area of FA increased with stiffness, and its values were similar to those reported for local tangential forces acting on individual FAs. These results reveal how the 3D cell shape feeds back on its internal organization and how it may control cell fate through FA-based signaling.
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