Evidence map›Paper›PMID 25157120›Full record

ArticleThe American journal of tropical medicine and hygiene2014

Genetic determinants of differential oral infection phenotypes of West Nile and St. Louis encephalitis viruses in Culex spp. mosquitoes.

Payal D Maharaj, Bethany G Bolling, Michael Anishchenko, William K Reisen, Aaron C Brault

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.3field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Comparative Vector Competence of North AmericanThe American journal of tropical medicine and hygiene · 2018
    Article
  6. Article
  7. Article
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Payal D MaharajDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Center for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California.
Bethany G BollingDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Center for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California.
Michael AnishchenkoDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Center for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California.
William K ReisenDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Center for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California.
Aaron C BraultDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Center for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California abrault@cdc.gov.
Centers for Disease Control and Prevention · US

Funding

Vaccines and Diagnostics TrialsU54AI065359 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI BARBOUR, ALAN G. · 2005 to 2013
$88.8M
Persistence of West Nile virus in CaliforniaR01AI055607 · NIAID · UNIVERSITY OF CALIFORNIA DAVIS · PI REISEN, WILLIAM K · 2003 to 2012
$4.4M
West Nile Viral Determinants of Avian PathogenesisR01AI061822 · NIAID · UNIVERSITY OF CALIFORNIA DAVIS · PI BRAULT, AARON COLE · 2004 to 2007
$1.3M
West Nile Virus Genotypic Affect on Host CompetenceR01CI000235 · CI · UNIVERSITY OF CALIFORNIA DAVIS · PI BRAULT, AARON COLE · 2004 to 2006
$181k
NCPDCID CDC HHS CI000235NCPDCID CDC HHS R01 CI000235NIAID NIH HHS AI061822NIAID NIH HHS AI065359NIAID NIH HHS AI55607NIAID NIH HHS R01 AI055607NIAID NIH HHS R01 AI061822NIAID NIH HHS U54 AI065359
6 · The paper itself

Abstract

St. Louis encephalitis virus (SLEV) has shown greater susceptibility to oral infectivity than West Nile virus (WNV) in Culex mosquitoes. To identify the viral genetic elements that modulate these disparate phenotypes, structural chimeras (WNV-pre-membrane [prM] and envelope [E] proteins [prME]/SLEV.IC (infectious clone) and SLEV-prME/WNV.IC) were constructed in which two of the structural proteins, the prM and E, were interchanged between viruses. Oral dose-response assessment with the chimeric/parental WNV and SLEV was performed to characterize the infection phenotypes in Culex mosquitoes by artificial blood meals. The median infectious dose required to infect 50% of Cx. quinquefasciatus with WNV was indistinguishable from that of the SLEV-prME/WNV.IC chimeric virus. Similarly, SLEV and WNV-prME/SLEV.IC virus exhibited an indistinguishable oral dose-response relationship in Cx. quinquefasciatus. Infection rates for WNV.IC and SLEV-prME/WNV.IC were significantly lower than SLEV.IC and WNV-prME/SLEV.IC infection rates. These results indicated that WNV and SLEV oral infectivities are not mediated by genetic differences within the prM and E proteins.

Indexed as

AnimalsCulexCulicidaeEncephalitis Virus, St. LouisFemalePhenotypeViral ProteinsWest Nile virusViral Proteins

Identifiers

PMID25157120
PMCPMC4228875
OpenAlexW2314879767

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.