ArticleJournal of bacteriology2014
Hydrogen formation and its regulation in Ruminococcus albus: involvement of an electron-bifurcating [FeFe]-hydrogenase, of a non-electron-bifurcating [FeFe]-hydrogenase, and of a putative hydrogen-sensing [FeFe]-hydrogenase.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 126 citations in OpenAlex.
- Individual variability in hydrogen-producing microbes influences the response to hydrogen supplementation on sleep quality: a randomized, double-blind, placebo-controlled, parallel study.Scientific reports · 2026Trial
- Formate- and Sodium Ion-Dependent Wood-Ljungdahl Pathway Involved in Organo-, Litho-, and Methylotrophic Growth of Tepidanaerobacter acetatoxydans.Environmental science & technology · 2026Article
- Rumen-derived Prevotella and Megasphaera elsdenii mitigate methane production through functional modulation of rumen microbial metabolism.Journal of animal science and biotechnology · 2026Article
- Reprogramming hydrogen metabolism for methane mitigation in dairy cows: mechanistic insights from polyphenols using meta-omics approaches.NPJ biofilms and microbiomes · 2026Article
- Adaptive evolution of electron transfer pathways inJournal of bacteriology · 2026Article
- Rumen-derived Pichia membranifaciens modulates the rumen microbiome and metabolome and mitigates methane emissions in dairy cows.NPJ biofilms and microbiomes · 2026Article
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- Carbohydrate-active enzyme architectures reflect distinct fungal and bacterial strategies for lignocellulose conversion to volatile fatty acids.ISME communications · 2026Article
- Investigating the antimethanogenic effects of selected nitro-compounds on methane production, rumen fermentation, and methanogenic archaeaApplied and environmental microbiology · 2025Article
- Short- and long-term dietary supplementation as well as withdrawal of the enteric methane inhibitor 3-nitrooxypropanol reveal distinct effects on the rumen microbial community.Journal of animal science and biotechnology · 2025Article
- A widespread hydrogenase supports fermentative growth of gut bacteria in healthy people.Nature microbiology · 2025Article
- Modeling reveals a metabolic basis of competition amongmSystems · 2025Article
- Methanogenesis inhibition remodels microbial fermentation and stimulates acetogenesis in ruminants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The role of AdhE mutations inJournal of bacteriology · 2025Article
- Genetic investigation of hydrogenases inApplied and environmental microbiology · 2025Article
- A reliable in vitro rumen culture system and workflow for screening anti-methanogenic compounds.PloS one · 2025Article
- The vast landscape of carbohydrate fermentation in prokaryotes.FEMS microbiology reviews · 2024Review
- Article
- - Invited Review - The role of rumen microbiota in enteric methane mitigation for sustainable ruminant production.Animal bioscience · 2024Article
- - Invited Review - Hydrogen production and hydrogen utilization in the rumen: key to mitigating enteric methane production.Animal bioscience · 2024Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ruminococcus albus 7 has played a key role in the development of the concept of interspecies hydrogen transfer. The rumen bacterium ferments glucose to 1.3 acetate, 0.7 ethanol, 2 CO2, and 2.6 H2 when growing in batch culture and to 2 acetate, 2 CO2, and 4 H2 when growing in continuous culture in syntrophic association with H2-consuming microorganisms that keep the H2 partial pressure low. The organism uses NAD(+) and ferredoxin for glucose oxidation to acetyl coenzyme A (acetyl-CoA) and CO2, NADH for the reduction of acetyl-CoA to ethanol, and NADH and reduced ferredoxin for the reduction of protons to H2. Of all the enzymes involved, only the enzyme catalyzing the formation of H2 from NADH remained unknown. Here, we report that R. albus 7 grown in batch culture on glucose contained, besides a ferredoxin-dependent [FeFe]-hydrogenase (HydA2), a ferredoxin- and NAD-dependent electron-bifurcating [FeFe]-hydrogenase (HydABC) that couples the endergonic formation of H2 from NADH to the exergonic formation of H2 from reduced ferredoxin. Interestingly, hydA2 is adjacent to the hydS gene, which is predicted to encode an [FeFe]-hydrogenase with a C-terminal PAS domain. We showed that hydS and hydA2 are part of a larger transcriptional unit also harboring putative genes for a bifunctional acetaldehyde/ethanol dehydrogenase (Aad), serine/threonine protein kinase, serine/threonine protein phosphatase, and a redox-sensing transcriptional repressor. Since HydA2 and Aad are required only when R. albus grows at high H2 partial pressures, HydS could be a H2-sensing [FeFe]-hydrogenase involved in the regulation of their biosynthesis.
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