ArticleAngewandte Chemie (International ed. in English)2014
Optogenetic engineering: light-directed cell motility.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- A revised model of nuclear actin import: Importin 9 competes with cofilin, profilin, and RanGTP for actin binding.bioRxiv : the preprint server for biology · 2025Article
- Turn-on protein switches for controlling actin binding in cells.Nature communications · 2024Article
- Quantifying the impact of electric fields on single-cell motility.Biophysical journal · 2021Article
- Tools of the trade: studying actin in zebrafish.Histochemistry and cell biology · 2020Review
- CofActor: A light- and stress-gated optogenetic clustering tool to study disease-associated cytoskeletal dynamics in living cells.The Journal of biological chemistry · 2020Article
- Light directed migration of a cluster of cells in the centimeter scale.Small GTPases · 2020Article
- Optogenetic control of cofilin and αTAT in living cells using Z-lock.Nature chemical biology · 2019Article
- Optogenetic perturbation of the biochemical pathways that control cell behavior.Methods in enzymology · 2019Article
- Design, construction, and validation of optogenetic proteins.Methods in enzymology · 2019Article
- Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase.Cell chemical biology · 2018Article
- Phototriggered Secretion of Membrane Compartmentalized Bioactive Agents.Angewandte Chemie (International ed. in English) · 2016Article
- Optogenetic apoptosis: light-triggered cell death.Angewandte Chemie (International ed. in English) · 2015Article
- Optimizing optogenetic constructs for control over signaling and cell behaviours.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Review
- Chemical biologists rush to San Francisco for the ICBS.Nature chemical biology · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Genetically encoded, light-activatable proteins provide the means to probe biochemical pathways at specific subcellular locations with exquisite temporal control. However, engineering these systems in order to provide a dramatic jump in localized activity, while retaining a low dark-state background remains a significant challenge. When placed within the framework of a genetically encodable, light-activatable heterodimerizer system, the actin-remodelling protein cofilin induces dramatic changes in the F-actin network and consequent cell motility upon illumination. We demonstrate that the use of a partially impaired mutant of cofilin is critical for maintaining low background activity in the dark. We also show that light-directed recruitment of the reduced activity cofilin mutants to the cytoskeleton is sufficient to induce F-actin remodeling, formation of filopodia, and directed cell motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.