ArticleInfection and immunity2014
Hydrogen peroxide production from glycerol metabolism is dispensable for virulence of Mycoplasma gallisepticum in the tracheas of chickens.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Epidemiology, Phylogenetic Divergence, and Differential Pathogenicity of Feline RespiratoryTransboundary and emerging diseases · 2026Article
- A sweeping view of avian mycoplasmas biology drawn from comparative genomic analyses.BMC genomics · 2025Article
- Unveiling genome plasticity and a novel phage inMicrobial genomics · 2024Article
- Comparative genomics ofMicrobial genomics · 2023Article
- Novel mycoplasma nucleomodulin MbovP475 decreased cell viability by regulating expression of CRYAB and MCF2L2.Virulence · 2022Article
- Review
- AApplied and environmental microbiology · 2021Article
- Multiple differences in pathogen-host cell interactions following a bacterial host shift.Scientific reports · 2020Article
- Proteases as Secreted Exoproteins in Mycoplasmas from Ruminant Lungs and Their Impact on Surface-Exposed Proteins.Applied and environmental microbiology · 2019Article
- Comparative evaluation of different therapeutic protocols for contagious caprine pleuropneumonia in Himalayan Pashmina goats.Tropical animal health and production · 2019Article
- Mycoplasma hyopneumoniae surface-associated proteases cleave bradykinin, substance P, neurokinin A and neuropeptide Y.Scientific reports · 2019Article
- Extracellular DNA: A Nutritional Trigger ofFrontiers in microbiology · 2019Article
- Removal of a Subset of Non-essential Genes Fully Attenuates a Highly VirulentFrontiers in microbiology · 2019Article
- Evidence for the Cytoplasmic Localization of the L-α-Glycerophosphate Oxidase in Members of the "Frontiers in microbiology · 2019Article
- Variable Lipoprotein Hemagglutinin A Gene (Infection and immunity · 2018Article
- Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and OScientific reports · 2017Article
- Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model.Scientific reports · 2016Article
- Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway.BMC microbiology · 2016Article
- Complete Genome Sequence of Mycoplasma flocculare Strain Ms42T (ATCC 27399T).Genome announcements · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen peroxide (H2O2) is a by-product of glycerol metabolism in mycoplasmas and has been shown to cause cytotoxicity for cocultured eukaryotic cells. There appears to be selective pressure for mycoplasmas to retain the genes needed for glycerol metabolism. This has generated interest and speculation as to their function during infection. However, the actual effects of glycerol metabolism and H2O2 production on virulence in vivo have never been assessed in any Mycoplasma species. To this end, we determined that the wild-type (WT) R(low) strain of the avian pathogen Mycoplasma gallisepticum is capable of producing H2O2 when grown in glycerol and is cytotoxic to eukaryotic cells in culture. Transposon mutants with mutations in the genes present in the glycerol transport and utilization pathway, namely, glpO, glpK, and glpF, were identified. All mutants assessed were incapable of producing H2O2 and were not cytotoxic when grown in glycerol. We also determined that vaccine strains ts-11 and 6/85 produce little to no H2O2 when grown in glycerol, while the naturally attenuated F strain does produce H2O2. Chickens were infected with one of two glpO mutants, a glpK mutant, R(low), or growth medium, and tracheal mucosal thickness and lesion scores were assessed. Interestingly, all glp mutants were reproducibly virulent in the respiratory tracts of the chickens. Thus, there appears to be no link between glycerol metabolism/H2O2 production/cytotoxicity and virulence for this Mycoplasma species in its natural host. However, it is possible that glycerol metabolism is required by M. gallisepticum in a niche that we have yet to study.
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