ArticleChembiochem : a European journal of chemical biology2014
Traceless semisynthesis of a set of histone 3 species bearing specific lysine methylation marks.
Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Core nucleosomes are refractory to lentiviral DNA integration.Nature communications · 2026Article
- Nucleosome context regulates chromatin reader preference.Nucleic acids research · 2025Article
- Advances in the chemical synthesis of human proteoforms.Science China. Life sciences · 2025Review
- Structural mechanism of H3K27 demethylation and crosstalk with heterochromatin markers.Molecular cell · 2025Article
- Beyond the tail: the consequence of context in histone post-translational modification and chromatin research.The Biochemical journal · 2024Article
- OaAEP1 Ligase-Assisted Chemoenzymatic Synthesis of Full Cysteine-Rich Metal-Binding Cyanobacterial Metallothionein SmtA.Bioconjugate chemistry · 2023Article
- Detection and Quantification of Histone Methyltransferase Activity In Vitro.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Chemoenzymatic Semisynthesis of Proteins.Chemical reviews · 2020Review
- The Use of Immunohistochemistry, FluorescenceFrontiers in oncology · 2020Review
- Chromatin structure and its chemical modifications regulate the ubiquitin ligase substrate selectivity of UHRF1.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Calibrating ChIP-Seq with Nucleosomal Internal Standards to Measure Histone Modification Density Genome Wide.Molecular cell · 2015Article
- Chemical and biological tools for the preparation of modified histone proteins.Topics in current chemistry · 2015Article
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Authors and funding
3 authors.
Funding
Abstract
Considerable mechanistic insight into the function of histone post-translational modifications and the enzymes that install and remove them derives from in vitro experiments with modified histones, often embedded in nucleosomes. We report the first semisyntheses of native-like histone 3 (H3) bearing tri- and dimethyllysines at position 79 and trimethyllysine at position 36, as well as more facile and traceless semisyntheses of K9 and K27 trimethylated species. These semisyntheses are practical on a multi-milligram scale and can also generate H3 with combinations of marks. Each of these modifications has distinct functional consequences, although the pathways by which H3K36me3 and H3K79me2/3 act have not been entirely mapped. To this end, we demonstrated that our semisynthetic histones, when reconstituted into nucleosomes, are valuable affinity reagents for unbiased binding partner discovery and compare them to their methyllysine analogue (MLA) counterparts at the nucleosome level.
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