ArticleMolecular microbiology2014
Proteomic profiling of a robust Wolbachia infection in an Aedes albopictus mosquito cell line.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Cycloheximide, a specific inhibitor of eukaryotic protein synthesis, enhances infection of mosquito cells with Wolbachia.In vitro cellular & developmental biology. Animal · 2026Article
- Stage-Specific Proteomic Adaptations to Heme-Induced Oxidative Stress inInternational journal of molecular sciences · 2026Article
- Resistance ofInsects · 2024Article
- A comprehensive review ofFrontiers in immunology · 2024Review
- From Mosquito Ovaries to Ecdysone; from Ecdysone toInsects · 2022Review
- DNA recombination and repair in Wolbachia: RecA and related proteins.Molecular genetics and genomics : MGG · 2021Article
- Intracellular Interactions Between Arboviruses andFrontiers in cellular and infection microbiology · 2021Review
- Wolbachia and Sirtuin-4 interaction is associated with alterations in host glucose metabolism and bacterial titer.PLoS pathogens · 2020Article
- Review
- Wolbachia pipientis grows in Saccharomyces cerevisiae evoking early death of the host and deregulation of mitochondrial metabolism.MicrobiologyOpen · 2019Article
- Detection of Plasmodium falciparum infected Anopheles gambiae using near-infrared spectroscopy.Malaria journal · 2019Article
- Strain-specific response to ampicillin in Wolbachia-infected mosquito cell lines.In vitro cellular & developmental biology. Animal · 2018Article
- Proteomic analysis of a mosquito host cell response to persistent Wolbachia infection.Research in microbiology · 2017Article
- Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon.BMC genomics · 2017Article
- Reliance ofGenetics · 2017Article
- Genomes of Candidatus Wolbachia bourtzisii wDacA and Candidatus Wolbachia pipientis wDacB from the Cochineal Insect Dactylopius coccus (Hemiptera: Dactylopiidae).G3 (Bethesda, Md.) · 2016Article
- Mosaic composition of ribA and wspB genes flanking the virB8-D4 operon in the Wolbachia supergroup B-strain, wStr.Archives of microbiology · 2016Article
- The Wolbachia WO bacteriophage proteome in the Aedes albopictus C/wStr1 cell line: evidence for lytic activity?In vitro cellular & developmental biology. Animal · 2016Article
- Dynamics of Wolbachia pipientis Gene Expression Across the Drosophila melanogaster Life Cycle.G3 (Bethesda, Md.) · 2015Article
- Effects of mimosine on Wolbachia in mosquito cells: cell cycle suppression reduces bacterial abundance.In vitro cellular & developmental biology. Animal · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Wolbachia pipientis, a widespread vertically transmitted intracellular bacterium, provides a tool for insect control through manipulation of host-microbe interactions. We report proteomic characterization of wStr, a Wolbachia strain associated with a strong cytoplasmic incompatibility phenotype in its native host, Laodelphax striatellus. In the Aedes albopictus C/wStr1 mosquito cell line, wStr maintains a robust, persistent infection. MS/MS analyses of gel bands revealed a protein 'footprint' dominated by Wolbachia-encoded chaperones, stress response and cell membrane proteins, including the surface antigen WspA, a peptidoglycan-associated lipoprotein and a 73 kDa outer membrane protein. Functional classifications and estimated abundance levels of 790 identified proteins suggested that expression, stabilization and secretion of proteins predominate over bacterial genome replication and cell division. High relative abundances of cysteine desulphurase, serine/glycine hydroxymethyl transferase, and components of the α-ketoglutarate dehydrogenase complex in conjunction with above average abundances of glutamate dehydrogenase and proline utilization protein A support Wolbachia genome-based predictions for amino acid metabolism as a primary energy source. wStr expresses 15 Vir proteins of a Type IV secretion system and its transcriptional regulator. Proteomic characterization of a robust insect-associated Wolbachia strain provides baseline information that will inform further development of in vitro protocols for Wolbachia manipulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.