Evidence map›Paper›PMID 25154385›Full record

ArticleElectrophoresis2014

Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics.

Max A Fahrenkopf, Tamal Mukherjee, B Erik Ydstie, James W Schneider

Abstract read
In one paragraph

Article in Electrophoresis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Max A FahrenkopfDepartment of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Tamal Mukherjee
B Erik Ydstie
James W Schneider

Funding

Simulation Tool to Rapidly Design Optimize and Prototype Microfluidic DevicesR44HG004290 · NHGRI · CFD RESEARCH CORPORATION · PI WANG, YI · 2009 to 2010
$803k
NHGRI NIH HHS 2R44HG00429002NHGRI NIH HHS R44 HG004290
6 · The paper itself

Abstract

We present a nonlinear optimization study of different implementations of the DNA electrophoretic method "End-labeled Free-solution Electrophoresis" in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro-osmotic counterflows and snap-shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags available in micelle form, we identify a design capable of sequencing 600 bases in 2.8 min.

Indexed as

DNAElectrophoresis, CapillarySequence Analysis, DNATime FactorsDNADNA electrophoresisDNA sequencingELFSEGel-freeRapid

Identifiers

PMID25154385
PMCPMC4504435

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.