ArticlePloS one2014
Modulation of biofilm-formation in Salmonella enterica serovar Typhimurium by the periplasmic DsbA/DsbB oxidoreductase system requires the GGDEF-EAL domain protein STM3615.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
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- Previously Uncharacterised Aliphatic Amino Acid Positions Modulate the Apparent Catalytic Activity of the EAL Domain of ZMO_1055 and Other Cyclic Di-GMP-Specific EAL Phosphodiesterases.Microbial biotechnology · 2026Article
- ProQ prevents mRNA degradation through inhibition of poly(A) polymerase.Nucleic acids research · 2025Article
- Raspberry Ketone-Mediated Inhibition of Biofilm Formation inAntibiotics (Basel, Switzerland) · 2023Article
- Comparison of the genetic basis of biofilm formation betweenMicrobial genomics · 2022Article
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- The Wsp system ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Yin and Yang of Biofilm Formation and Cyclic di-GMP Signaling of the Gastrointestinal Pathogen Salmonella enterica Serovar Typhimurium.Journal of innate immunity · 2022Review
- Massively parallel transposon mutagenesis identifies temporally essential genes for biofilm formation inMicrobial genomics · 2021Article
- Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification.BMC biotechnology · 2021Article
- Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study.Journal of applied biomedicine · 2020Article
- Salmonella and Reactive Oxygen Species: A Love-Hate Relationship.Journal of innate immunity · 2019Review
- CpxR/CpxA ControlsJournal of bacteriology · 2018Article
- The curli regulator CsgD mediates stationary phase counter-silencing of csgBA in Salmonella Typhimurium.Molecular microbiology · 2018Article
- Redox-active conducting polymers modulateNPJ biofilms and microbiomes · 2017Article
- HmsC ControlsFrontiers in cellular and infection microbiology · 2017Article
- Genetic control of bacterial biofilms.Journal of applied genetics · 2016Review
- Novel Two-Step Hierarchical Screening of Mutant Pools Reveals Mutants under Selection in Chicks.Infection and immunity · 2016Article
- Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner.Applied and environmental microbiology · 2016Article
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Authors and funding
4 authors at 1 institution in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
In Salmonella enterica serovar Typhimurium (S. Typhimurium), biofilm-formation is controlled by the cytoplasmic intracellular small-molecular second messenger cyclic 3', 5'-di- guanosine monophosphate (c-di-GMP) through the activities of GGDEF and EAL domain proteins. Here we describe that deleting either dsbA or dsbB, respectively encoding a periplasmic protein disulfide oxidase and a cytoplasmic membrane disulfide oxidoreductase, resulted in increased biofilm-formation on solid medium. This increased biofilm-formation, defined as a red, dry and rough (rdar) colony morphotype, paralleled with enhanced expression of the biofilm master regulator CsgD and the biofilm-associated fimbrial subunit CsgA. Deleting csgD in either dsb mutant abrogated the enhanced biofilm-formation. Likewise, overexpression of the c-di-GMP phosphodiesterase YhjH, or mutationally inactivating the CsgD activator EAL-domain protein YdiV, reduced biofilm-formation in either of the dsb mutants. Intriguingly, deleting the GGDEF-EAL domain protein gene STM3615 (yhjK), previously not connected to rdar morphotype development, also abrogated the escalated rdar morphotype formation in dsb mutant backgrounds. Enhanced biofilm-formation in dsb mutants was furthermore annulled by exposure to the protein disulfide catalyst copper chloride. When analyzed for the effect of exogenous reducing stress on biofilm-formation, both dsb mutants initially showed an escalated rdar morphotype development that later dissolved to reveal a smooth mucoid colony morphotype. From these results we conclude that biofilm-development in S. Typhimurium is affected by periplasmic protein disulphide bond status through CsgD, and discuss the involvement of selected GGDEF/EAL domain protein(s) as signaling mediators.
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