ArticlePloS one2014
Nuclear glycolytic enzyme enolase of Toxoplasma gondii functions as a transcriptional regulator.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review and meta-analysis of variation in Toxoplasma gondii cyst burden in the murine model.Experimental parasitology · 2019Pooled it
- Activity Evaluation and Mode of Action of ICA AgainstBiomolecules · 2025Article
- Exploring glycolytic enzymes in disease: potential biomarkers and therapeutic targets in neurodegeneration, cancer and parasitic infections.Open biology · 2025Review
- Tumour-specific phosphorylation of serine 419 drives alpha-enolase (ENO1) nuclear export in triple negative breast cancer progression.Cell & bioscience · 2024Article
- Two apicoplast dwelling glycolytic enzymes provide key substrates for metabolic pathways in the apicoplast and are critical for Toxoplasma growth.PLoS pathogens · 2022Article
- Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii.BMC research notes · 2022Article
- The determinants regulatingFrontiers in microbiology · 2022Review
- Nuclear dihydroxyacetone phosphate signals nutrient sufficiency and cell cycle phase to global histone acetylation.Nature metabolism · 2021Article
- Lysine crotonylation is widespread on proteins of diverse functions and localizations in Toxoplasma gondii.Parasitology research · 2021Article
- Dehydroepiandrosterone Effect onMicroorganisms · 2021Article
- Moonlighting Proteins: The Case of the Hexokinases.Frontiers in molecular biosciences · 2021Review
- Global profiling of lysine 2-hydroxyisobutyrylome in Toxoplasma gondii using affinity purification mass spectrometry.Parasitology research · 2020Article
- Alpha-enolase in viral target cells suppresses the human immunodeficiency virus type 1 integration.Retrovirology · 2020Article
- Toxoplasma gondii metacaspase 2 is an important factor that influences bradyzoite formation in the Pru strain.Parasitology research · 2020Article
- TheBiology open · 2019Article
- ENOblock inhibits the pathology of diet-induced obesity.Scientific reports · 2019Article
- Gene target discovery with network analysis in Toxoplasma gondii.Scientific reports · 2019Article
- A proteomic portrait of dinoflagellate chromatin reveals abundant RNA-binding proteins.Chromosoma · 2018Article
- Recombinant Enolase ofJournal of immunology research · 2018Article
- Serodiagnostic Potential of Alpha-Enolase FromFrontiers in microbiology · 2018Article
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7 authors.
Funding
Abstract
Apicomplexan parasites including Toxoplasma gondii have complex life cycles within different hosts and their infectivity relies on their capacity to regulate gene expression. However, little is known about the nuclear factors that regulate gene expression in these pathogens. Here, we report that T. gondii enolase TgENO2 is targeted to the nucleus of actively replicating parasites, where it specifically binds to nuclear chromatin in vivo. Using a ChIP-Seq technique, we provide evidence for TgENO2 enrichment at the 5' untranslated gene regions containing the putative promoters of 241 nuclear genes. Ectopic expression of HA-tagged TgENO1 or TgENO2 led to changes in transcript levels of numerous gene targets. Targeted disruption of TgENO1 gene results in a decrease in brain cyst burden of chronically infected mice and in changes in transcript levels of several nuclear genes. Complementation of this knockout mutant with ectopic TgENO1-HA fully restored normal transcript levels. Our findings reveal that enolase functions extend beyond glycolytic activity and include a direct role in coordinating gene regulation in T. gondii.
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