ArticleZebrafish2014
High-throughput analysis of behavior in zebrafish larvae: effects of feeding.
Article in Zebrafish, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 81 citations in OpenAlex.
- Brewing Anxiety: Caffeine-Induced Thigmotaxis in Zebrafish Larvae.Brain and behavior · 2026Article
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- Time-Resolved Proteomic Analysis in Zebrafish Using Bioorthogonal Noncanonical Amino Acid Tagging.Journal of proteome research · 2026Article
- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.Current biology : CB · 2026Article
- Diquat based herbicide impair the development and behavior of zebrafish embryos and larvae.Scientific reports · 2025Article
- Article
- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.bioRxiv : the preprint server for biology · 2025Article
- Zebrafish as a Model Organism for Post-Traumatic Stress Disorder: Insights into Stress Mechanisms and Behavioral Assays.Biology · 2025Review
- Target the Heart: A New Axis of Alzheimer's Disease Prevention.Journal of dementia and alzheimer's disease · 2025Article
- Zebrafishology, study design guidelines for rigorous and reproducible data using zebrafish.Communications biology · 2025Review
- Marigold: a machine learning-based web app for zebrafish pose tracking.BMC bioinformatics · 2025Article
- Early disruptions in vitamin D receptor signaling induces persistent developmental behavior deficits in zebrafish larvae.PloS one · 2025Article
- High throughput machine learning pipeline to characterize larval zebrafish motor behavior.PloS one · 2025Article
- A high-throughput system for drug screening based on the movement analysis of zebrafish.Heliyon · 2024Article
- Behavioral Effects of the Mixture and the Single Compounds Carbendazim, Fipronil, and Sulfentrazone on Zebrafish (Biomedicines · 2024Article
- Differential Roles of Diet on Development and Spinal Cord Regeneration in Larval Zebrafish.Zebrafish · 2024Article
- Drug repurposing for neurodegenerative diseases using Zebrafish behavioral profiles.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2024Article
- Behavioral effects of visual stimuli in adult zebrafish using a novel eight-tank imaging system.Frontiers in behavioral neuroscience · 2024Article
- Diving deep: zebrafish models in motor neuron degeneration research.Frontiers in neuroscience · 2024Review
- Optimizing assays of zebrafish larvae swimming performance for drug discovery.Expert opinion on drug discovery · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Early brain development can be influenced by numerous genetic and environmental factors, with long-lasting effects on brain function and behavior. Identification of these factors is facilitated by high-throughput analyses of behavior in zebrafish larvae, which can be imaged in multiwell or multilane plates. However, the nutritional needs of zebrafish larvae during the behavioral experiments are not fully understood. Zebrafish larvae begin feeding between 4 and 5 days postfertilization (dpf), but can live solely on nutrients derived from the yolk until at least 7 dpf. To examine whether feeding affects behavior, we measured a broad range of behaviors with and without feeding at 5, 6, and 7 dpf. We found that feeding did not have a significant effect on behavior in 5-day-old larvae. In contrast, fed 6- and 7-day-old larvae displayed increased avoidance responses to visual stimuli, increased swim speeds, and decreased resting in comparison to unfed larvae. In addition, the fed 7-day-old larvae displayed a decrease in thigmotaxis and a decrease in the distance between larvae in the presence of visual stimuli. Thus, feeding affects a range of behaviors in 6- and 7-day-old larvae. We conclude that 5-day-old larvae are well-suited for high-throughput analyses of behavior, since effects of feeding can be avoided at this time. For high-throughput analyses of behavior in older larvae, standard feeding protocols need to be developed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.