ArticleFrontiers in bioengineering and biotechnology2014
Improved Free Fatty Acid Production in Cyanobacteria with Synechococcus sp. PCC 7002 as Host.
Article in Frontiers in bioengineering and biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 180 citations in OpenAlex.
- Towards sustainable production of biofuels via milking the cells of Synechococcus elongatus PCC 7942 engineered for free fatty acid excretion.Biotechnology for biofuels and bioproducts · 2026Article
- Overexpression of endogenous galactolipases and an efflux transporter enhances the secretion of extracellular free fatty acids by Synechococcus elongatus PCC 7942.Biotechnology for biofuels and bioproducts · 2026Article
- Engineering Cyanobacteria for High-Yield Photosynthetic Isoprene Production With Long-Term Phenotypic Stability.Plant biotechnology journal · 2026Article
- Astaxanthin Overproduction Enhanced by Metabolomics-Guided Rational Metabolic Engineering inACS synthetic biology · 2025Article
- Article
- High-density CRISPRi screens reveal diverse routes to improved acclimation in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Biotechnological Applications of Cyanobacteria: Synechocystis and Synechococcus Strains.Advances in biochemical engineering/biotechnology · 2025Review
- Triple-Gene Overexpression of the AcrA-AcrB-TolC Transporter System inInternational journal of molecular sciences · 2024Article
- Insufficient Acetyl-CoA Pool Restricts the Phototrophic Production of Organic Acids in Model Cyanobacteria.International journal of molecular sciences · 2024Review
- Nutraceutical prospects of genetically engineered cyanobacteria- technological updates and significance.World journal of microbiology & biotechnology · 2024Review
- Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene inInternational journal of molecular sciences · 2024Article
- RuBisCO activity assays: a simplified biochemical redox approach for in vitro quantification and an RNA sensor approach for in vivo monitoring.Microbial cell factories · 2024Article
- Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities.Biotechnology for biofuels and bioproducts · 2024Review
- A Novel Strain of the Cyanobacterial Growth-promoting Bacterium, Rhodococcus sp. AF2108, Enhances the Growth of Synechococcus elongatus.Microbes and environments · 2024Article
- Improved lipid production and component of mycosporine-like amino acids by co-overexpression of amt1 and aroB genes in Synechocystis sp. PCC6803.Scientific reports · 2023Article
- Increased Biomass and Polyhydroxybutyrate Production byInternational journal of molecular sciences · 2023Article
- Incorporation, fate, and turnover of free fatty acids in cyanobacteria.FEMS microbiology reviews · 2023Review
- Advances in Genetic Engineering in Improving Photosynthesis and Microalgal Productivity.International journal of molecular sciences · 2023Review
- Metabolic Engineering Design Strategies for Increasing Carbon Fluxes Relevant for Biosynthesis in Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- Introduction to Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
1 author at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Microbial free fatty acids (FFAs) have been proposed as a potential feedstock for renewable energy. The ability to directly convert carbon dioxide into FFAs makes cyanobacteria ideal hosts for renewable FFA production. Previous metabolic engineering efforts using the cyanobacterial hosts Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942 have demonstrated this direct conversion of carbon dioxide into FFAs; however, FFA yields in these hosts are limited by the negative impact of FFA production on the host cell physiology. This work investigates the use of Synechococcus sp. PCC 7002 as a cyanobacterial host for FFA production. In comparison to S. elongatus PCC 7942, Synechococcus sp. PCC 7002 strains produced and excreted FFAs at similar concentrations but without the detrimental effects on host physiology. The enhanced tolerance to FFA production with Synechococcus sp. PCC 7002 was found to be temperature-dependent, with physiological effects such as reduced photosynthetic yield and decreased photosynthetic pigments observed at higher temperatures. Additional genetic manipulations were targeted for increased FFA production, including thioesterases and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Overexpression of non-native RuBisCO subunits (rbcLS) from a psbAI promoter resulted in more than a threefold increase in FFA production, with excreted FFA concentrations reaching >130 mg/L. This work illustrates the importance of host strain selection for cyanobacterial biofuel production and demonstrates that the FFA tolerance of Synechococcus sp. PCC 7002 can allow for high yields of excreted FFA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.