ArticleBiochemical and biophysical research communications2014
Ascorbic acid prevents high glucose-induced apoptosis in human brain pericytes.
Article in Biochemical and biophysical research communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Diet and risk of diabetic retinopathy: a systematic review.European journal of epidemiology · 2018Pooled it
- Establishment of Three Different Glycation-Damage Cell Models and Analysis of Their Action Mechanism.Metabolites · 2026Article
- Targeting the AGEs-RAGE axis: pathogenic mechanisms and therapeutic interventions in diabetic wound healing.Frontiers in medicine · 2025Review
- Metabolic-driven analytics of traumatic brain injury and neuroprotection by ethyl pyruvate.Journal of neuroinflammation · 2024Article
- Pericyte Loss in Diseases.Cells · 2023Review
- Low Levels of Vitamin C during Pregnancy; a Risk Marker of Progression of Diabetic Retinopathy in Type 1 Diabetic Women?Antioxidants (Basel, Switzerland) · 2023Article
- The Blood-Brain Barrier, Oxidative Stress, and Insulin Resistance.Antioxidants (Basel, Switzerland) · 2021Review
- Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2021Article
- Healthy aging and the blood-brain barrier.Nature aging · 2021Review
- Pericytes: Intrinsic Transportation Engineers of the CNS Microcirculation.Frontiers in physiology · 2021Review
- CoQJournal of clinical medicine · 2020Article
- Green synthesis of silver nanoparticles bySaudi journal of biological sciences · 2020Article
- Cadmium-Induced Oxidative Stress: Focus on the Central Nervous System.Antioxidants (Basel, Switzerland) · 2020Review
- Adjuvant Therapies in Diabetic Retinopathy as an Early Approach to Delay Its Progression: The Importance of Oxidative Stress and Inflammation.Oxidative medicine and cellular longevity · 2020Review
- Oxidative Stress as the Main Target in Diabetic Retinopathy Pathophysiology.Journal of diabetes research · 2019Review
- Role and Molecular Mechanisms of Pericytes in Regulation of Leukocyte Diapedesis in Inflamed Tissues.Mediators of inflammation · 2019Review
- Impact of Metabolic Diseases on Cerebral Circulation: Structural and Functional Consequences.Comprehensive Physiology · 2018Review
- Vitamin C Improves Gastroparesis in Diabetic Rats: Effects on Gastric Contractile Responses and Oxidative Stress.Digestive diseases and sciences · 2017Article
- Review
- Ascorbic acid repletion: A possible therapy for diabetic macular edema?Free radical biology & medicine · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
High glucose concentrations due to diabetes increase apoptosis of vascular pericytes, impairing vascular regulation and weakening vessels, especially in brain and retina. We sought to determine whether vitamin C, or ascorbic acid, could prevent such high glucose-induced increases in pericyte apoptosis. Culture of human microvascular brain pericytes at 25 mM compared to 5mM glucose increased apoptosis measured as the appearance of cleaved caspase 3. Loading the cells with ascorbate during culture decreased apoptosis, both at 5 and 25 mM glucose. High glucose-induced apoptosis was due largely to activation of the receptor for advanced glycation end products (RAGE), since it was prevented by specific RAGE inhibition. Culture of pericytes for 24h with RAGE agonists also increased apoptosis, which was completely prevented by inclusion of 100 μM ascorbate. Ascorbate also prevented RAGE agonist-induced apoptosis measured as annexin V binding in human retinal pericytes, a cell type with relevance to diabetic retinopathy. RAGE agonists decreased intracellular ascorbate and GSH in brain pericytes. Despite this evidence of increased oxidative stress, ascorbate prevention of RAGE-induced apoptosis was not mimicked by several antioxidants. These results show that ascorbate prevents pericyte apoptosis due RAGE activation. Although RAGE activation decreases intracellular ascorbate and GSH, the prevention of apoptosis by ascorbate may involve effects beyond its function as an antioxidant.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.