ArticleStem cell research2014
The organoid-initiating cells in mouse pancreas and liver are phenotypically and functionally similar.
Article in Stem cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Organoids of liver diseases: From bench to bedside.World journal of gastroenterology · 2019Pooled it
- Adult canine pancreatic organoids enable functional analysis of pancreatic epithelial barrier and inflammatory responses.BMC molecular and cell biology · 2026Article
- Review
- Identification of a Novel Subset of Human Airway Epithelial Basal Stem Cells.International journal of molecular sciences · 2024Article
- An Exploration of Organoid Technology: Present Advancements, Applications, and Obstacles.Current pharmaceutical biotechnology · 2024Review
- Morphogenesis and development of human telencephalic organoids in the absence and presence of exogenous extracellular matrix.The EMBO journal · 2023Article
- Methylcellulose colony assay and single-cell micro-manipulation reveal progenitor-like cells in adult human pancreatic ducts.Stem cell reports · 2023Article
- Cellular and transcriptional heterogeneity in the intrahepatic biliary epithelium.Gastro hep advances · 2023Article
- "Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry.Analytical chemistry · 2022Article
- Long-Term Characteristics of Human-Derived Biliary Organoids under a Single Continuous Culture Condition.Cells · 2022Article
- Microfluidics Formulated Liposomes of Hypoxia Activated Prodrug for Treatment of Pancreatic Cancer.Pharmaceutics · 2022Article
- Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity.STAR protocols · 2022Article
- Proteomic analysis distinguishes extracellular vesicles produced by cancerous versus healthy pancreatic organoids.Scientific reports · 2022Article
- Engineering Hydrogels for the Development of Three-Dimensional In Vitro Models.International journal of molecular sciences · 2022Review
- Pancreatic Organoids for Regenerative Medicine and Cancer Research.Frontiers in cell and developmental biology · 2022Review
- Towards organoid culture without Matrigel.Communications biology · 2021Review
- Review
- Review
- A pancreas tumor derived organoid study: from drug screen to precision medicine.Cancer cell international · 2021Review
- Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Pancreatic Lgr5 expression has been associated with organoid-forming epithelial progenitor populations but the identity of the organoid-initiating epithelial cell subpopulation has remained elusive. Injury causes the emergence of an Lgr5(+) organoid-forming epithelial progenitor population in the adult mouse liver and pancreas. Here, we define the origin of organoid-initiating cells from mouse pancreas and liver prior to Lgr5 activation. This clonogenic population was defined as MIC1-1C3(+)/CD133(+)/CD26(-) in both tissues and the frequency of organoid initiation within this population was approximately 5% in each case. The transcriptomes of these populations overlapped extensively and showed enrichment of epithelial progenitor-associated regulatory genes such as Sox9 and FoxJ1. Surprisingly, pancreatic organoid cells also had the capacity to generate hepatocyte-like cells upon transplantation to Fah(-/-) mice, indicating a differentiation capacity similar to hepatic organoids. Although spontaneous endocrine differentiation of pancreatic progenitors was not observed in culture, adenoviral delivery of fate-specifying factors Pdx1, Neurog3 and MafA induced insulin expression without glucagon or somatostatin. Pancreatic organoid cultures therefore preserve many key attributes of progenitor cells while allowing unlimited expansion, facilitating the study of fate determination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.