ReviewHistochemistry and cell biology2014
Antibodies for immunolabeling by light and electron microscopy: not for the faint hearted.
Review in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
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- Prostate-specific antigen: An unfamiliar protein in the human salivary glands.Journal of anatomy · 2024Article
- Spatial activity mapping of ß-mannanase on soybean seeds.Scientific reports · 2024Article
- The Compartmentalization of Amyloid-β in Idiopathic Normal Pressure Hydrocephalus Brain Biopsies.Journal of Alzheimer's disease : JAD · 2024Article
- Extracting nanoscale membrane morphology from single-molecule localizations.Biophysical journal · 2023Article
- Gold Particle Analyser: Detection and quantitative assessment of electron microscopy gold probes.PloS one · 2023Article
- NRas activity is regulated by dynamic interactions with nanoscale signaling clusters at the plasma membrane.iScience · 2022Article
- Approach to map nanotopography of cell surface receptors.Communications biology · 2022Article
- Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins.The Journal of cell biology · 2022Article
- Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale.Frontiers in cell and developmental biology · 2022Review
- Just Seeing Is Not Enough for Believing: Immunolabelling as Indisputable Proof of SARS-CoV-2 Virions in Infected Tissue.Viruses · 2021Article
- The Trypanosoma brucei subpellicular microtubule array is organized into functionally discrete subdomains defined by microtubule associated proteins.PLoS pathogens · 2021Article
- MEK Inhibition Reverses Aberrant Signaling in Melanoma Cells through Reorganization of NRas and BRAF in Self Nanoclusters.Cancer research · 2021Article
- Article
- Computational Methods for Single-Cell Imaging and Omics Data Integration.Frontiers in molecular biosciences · 2021Review
- Concentrative Nucleoside Transporter 3 Is Located on Microvilli of Vaginal Epithelial Cells.ACS omega · 2020Article
- Localization of ZIP14 and ZIP8 in HIBCPP Cells.Brain sciences · 2020Article
- Article
- Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.Bio-protocol · 2019Article
- Correlative single-molecule localization microscopy and electron tomography reveals endosome nanoscale domains.Traffic (Copenhagen, Denmark) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Reliable antibodies represent crucial tools in the arsenal of the cell biologist and using them to localize antigens for immunocytochemistry is one of their most important applications. However, antibody-antigen interactions are much more complex and unpredictable than suggested by the old 'lock and key' analogy, and the goal of trying to prove that an antibody is specific is far more difficult than is generally appreciated. Here, we discuss the problems associated with the very complicated issue of trying to establish that an antibody (and the results obtained with it) is specific for the immunolabeling approaches used in light or electron microscopy. We discuss the increasing awareness that significant numbers of commercial antibodies are often not up to the quality required. We provide guidelines for choosing and testing antibodies in immuno-EM. Finally, we describe how quantitative EM methods can be used to identify reproducible patterns of antibody labeling and also extract specific labeling distributions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.